Development of Engineered Micro-Particles via Spray Drying for Respiratory Drug Delivery

  • Author / Creator
    Matinkhoo, Sadaf
  • In this study, respirable dry powders for the treatment of Pseudomonas aeruginosa and Burkholderia cepacia complex infections in cystic fibrosis patients were developed. The feasibility of spray drying aqueous solutions containing bacteriophages was investigated. Bacteriophages KS4-M, KS14 and ФKZ/D3 cocktail (active against P. aeruginosa or Burkholderia cepacia) were spray dried in solutions containing other excipients including trehalose and L-leucine. Acceptable process loss (0.4-0.8 log pfu), high delivery efficiency (69.7% of capsule load for the lead formulation), and desirable particle size (2.5-2.8 μm MMAD) were achieved. Then, L-leucine was substituted by and tested against D-leucine in the lead formulation to help eradicate bacteria biofilms. Similar morphologies and MMADs (3.25±0.03 μm and 3.82±0.04 μm for D- and L-leucine/trehalose) were achieved. Both formulations had acceptable total lung mass fractions (62.0±7.81% and 63.66±16.37% of emitted mass for D- and L-leucine/trehalose). Substitution L-leucine with D-leucine was found to be feasible.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Mechanical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Dr. Reinhard Vehring, Department of Mechanical Engineering
  • Examining committee members and their departments
    • Dr. Aloke Kumar, Department of Mechanical Engineering
    • Dr. Warren H. Finlay, Department of Mechanical Engineering