Characterization of bacteriophage receptors in the Burkholderia cepacia complex (Bcc)

  • Author / Creator
    Juárez-Lara, Gerardo R.
  • Because of their ability to infect bacterial cells, bacteriophages or phages (viruses that infect and kill bacteria) show promise in treating diseases caused by multidrug resistant bacteria like the Burkholderia cepacia complex (Bcc). In order to carry out their lifecycle, phages must recognize, adsorb to, and inject their genetic material into the host. Host recognition is a crucial stage during phage infection and is divided in two main stages: a) reversible binding, characterized by low affinity binding of the phage to a secondary receptor in the outermost membrane of the host, and b) irreversible binding, characterized by a high affinity binding of the phage to its primary receptor. A wide variety of phage receptors have been characterized including lipopolysaccharide, pili, flagella, porins, capsules, and ligand-gated porins. Two random plasposon insertion libraries were screened against a collection of Bcc specific phages. Bacterial mutants resistant to phage infection were then isolated and the disrupted genes and proteins involved in phage adherence were characterized.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Biological Sciences
  • Specialization
    • Microbiology and biotechnology
  • Supervisor / co-supervisor and their department(s)
    • Dennis, Jonathan J. (Biological Sciences)
  • Examining committee members and their departments
    • Szymanski, Christine (Biological Sciences)
    • Feldman, Mario (Biological Sciences)
    • Pukatzki, Stefan (Medical Microbiology and Immunology)