Using Phage Display to Identify Peptides that Bind to the Surface of Helicobacter pylori

  • Author / Creator
    Seetharaman Srinivasan, Praveen Raj
  • Recent studies have reported high rates of Helicobacter pylori (H. pylori) infection and gastric disease in the remote community of Aklavik (NWT) as compared with urban centers in Alberta. Current therapies fail to eradicate 20-25% of H. pylori infections, which may be related to antimicrobial resistance, poor compliance and, perhaps, inadequate drug delivery strategies. Proteins and unknown outer surface molecules (OSM) found on the bacterial surface of H. pylori play a major role in colonization and pathogen-host interaction. A novel therapeutic that will specifically target the OSM of bacteria and kill them may provide an effective alternative for the eradication of H. pylori infections. To this end, it is essential to identify peptides that bind specifically to the OSM of H. pylori. Phage display library is a tool that can interact with OSM of bacteria by exposing a library of a specific phage, each displaying one kind of the PIII minor coat proteins on its surface. This thesis Identified 20 peptides that bind to the OSM of four isolates of H. pylori, which is a first step towards the development of a targeted treatment for H. pylori infection.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Medical Sciences- Laboratory Medicine and Pathology
  • Supervisor / co-supervisor and their department(s)
    • Monika Keelan, Laboratory Medicine and Pathology
  • Examining committee members and their departments
    • Jason Acker, Laboratory Medicine and Pathology, Examination Chair
    • Larry D. Unsworth, Chemical and Materials Engineering
    • Leluo Guan, Agriculture, Food and Nutritional Sciences
    • Locksley E. McGann, Laboratory Medicine and Pathology