The functional characterization of the SnRK1 protein complex in hybrid poplar

• Author / Creator

  Lam, Kimberley SW

• The sucrose-nonfermenting-1 related kinase 1 (SnRK1) protein complex is a heterotrimeric serine/threonine protein kinase complex conserved in eukaryotes that acts as a regulator of carbon metabolism and energy homeostasis. The objective of this study was to determine if the SnRK1 protein complex has a role in the nitrogen response and during dormancy acquisition in poplar. Gene expression profiling of the PtdSnRK1, PtdAKINbeta, and PtdAKINgamma gene family members was carried out using a robust qRT-PCR assay. A subset of these genes showed modified expression patterns under differential nitrogen availability and during dormancy acquisition, suggesting that SnRK1 complexes comprised of specific subunits may be involved in the regulation of the response to nitrogen and during dormancy acquisition. The regulatory subunits PtdAKINbeta1.1, PtdAKINgamma1.1 and PtdAKINgamma2.3 were often identified using principal component analysis as significantly responsible for distinguishing treatments from one another and therefore merit further study.

• Subjects / Keywords

  • SnRK1
  • Nitrogen
  • AKINgamma
  • Poplar
  • Expression profile
  • Dormancy
  • AKINbeta

• Graduation date

  Fall 2010

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R30D81

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.