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Maternal Overweight Prior to Pregnancy and its Impact on the Infant Gut Microbiome and Subsequent Child Overweight Risk

  • Author / Creator
    Kalu, Rose N
  • Background: Maternal overweight and obesity is a widespread problem in Canada that has been linked to many complications during pregnancy. The gut microbiome has been revealed to have origins during infancy, which might be influenced by maternal weight gain during pregnancy. It would be beneficial to study the microbiome as a possible link between maternal and child overweight risk. Objective: To assess the impact of maternal overweight, prior to pregnancy, on the infant gut microbiome and child overweight. Methods: Height and weight measurements of 1021 women, and their children were obtained from the birth chart in the Canadian Healthy Infant Longitudinal Development [CHILD] study. Information on the infant gut microbiome was acquired from fecal samples in diapers, where DNA was isolated with a commercial kit ‘Qiagen QIAamp DNA stool Mini Kit’, and extracted and amplified from the hypervariable V4 region of the 16S rRNA locus using a Miseq2 machine. Results: Maternal prepregnancy overweight doubled the risk of child overweight at one year, independent of mode of delivery, exclusive breast feeding or formula feeding, and infant antibiotic exposure by three months. Exclusive breastfeeding on the other hand, lowered the risk of child overweight by 33%. Maternal pregnancy overweight is associated with a low relative abundance of Bacteroidaceae in the infant gut microbiome,. Conclusions: Maternal pregnancy overweight doubles the risk of child overweight, and appears to lower the risk of the presence of Bacteroidaceae in the infant gut microbiome. These findings offer further evidence of the necessity of establishing preventive measures in clinical practice to halt the harmful sequelae of overweight and obesity in pregnancy.

  • Subjects / Keywords
  • Graduation date
    2015-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3XH4X
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Medical Sciences-Paediatrics
  • Supervisor / co-supervisor and their department(s)
    • Kozyrskyj, Anita (Pediatrics)
  • Examining committee members and their departments
    • Chari, Radha (Obstetrics and Gynecology)
    • Haqq, Andrea (Pediatrics)