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The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice
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DNA double-strand breaks (DSBs) are repaired mainly by non-homologous end
joining (NHEJ) or homologous recombination (HR). Cell cycle stage and DNA end
resection are believed to regulate the commitment to HR repair. Here we
identify RNF138 as an ubiquitin E3 ligase that regulates the HR pathway.
RNF138 is recruited to DNA damage sites through zinc fingers that have a strong
preference for DNA with 5’- or 3’- single-stranded overhangs. RNF138
stimulates DNA end resection and promotes ATR-dependent signalling and DSB
repair by HR, thereby contributing to cell survival upon exposure to DSBinducing agents. Finally, we establish that RNF138-dependent Ku removal from
DNA breaks is one mechanism whereby RNF138 can promote HR. These results
establish RNF138 as an important regulator of DSB repair pathway choice. -
- Date created
- 2015-01-01
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- Subjects / Keywords
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- Type of Item
- Article (Published)