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Single Chain Fraction Variable Binding Molecules as Bone-Targeting Therapeutics and Diagnostics

  • Author / Creator
    Lam, Michael
  • Osteoporosis is a disease characterized by lowering of bone mass and subsequent bone fracture. Although successful antiresorptive treatment options are commercially available, they have disadvantages such as poor bioavailability and significant side effects. Using phage display, an in vitro high throughput screening method, we sought to generate single chain fraction variable (scFv) against osteoclast surface receptors and bone turnover markers, for the evaluation of their potential as drug-targeted delivery platforms for improved bioavailability of current therapeutics and as immunodiagnostic assay reagents in osteoporosis. With our current in vitro result, it can be concluded that scFv, although having weaker binding affinity than IgG antibody, still possesses good selective binding against antigens. With this method of generating scFv being more cost-effective and less labour intensive, scFv reagents can become a viable option in site-directed drug delivery and immunodiagnostic for osteoporosis and possibly for cross application to other bone-modifying disease such as osteoarthritis.

  • Subjects / Keywords
  • Graduation date
    2011-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3FH1F
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Faculty of Pharmacy and Pharmaceutical Sciences
  • Supervisor / co-supervisor and their department(s)
    • Doschak, Michael (Pharmacy and Pharmaceutical Sciences)
  • Examining committee members and their departments
    • Jurasz, Paul (Pharmacy and Pharmaceutical Sciences)
    • El-Kadi, Ayman (Pharmacy and Pharmaceutical Sciences)
    • Kaur, Kamaljit (Pharmacy and Pharmaceutical Sciences)
    • Dixon, Walter (Agric, Food & Nutritional Science)