Linkage mapping, phenotypic characterization, and introgression analysis of crosses with safflower (Carthamus tinctorius)

• Author / Creator

  Archibald, Catherine J.

• An inter-specific linkage map of a backcross (BC1) population derived from a cross between C. tinctorius and C. oxyacanthus was developed using primarily microsatellite markers. A composite map, which aligned the inter-specific (C. tinctorius x C. oxyacanthus) map with an intra-specific linkage map previously developed from an F2 population of C. tinctorius, was created to search for syntenic regions. Results indicate that despite low marker saturation, there is substantial colinearity between the two linkage maps, and one translocation or inversion event. Upon subsequent self-fertilization events, phenotypes of the inter-specific backcross population were characterized in both the field (BC1S2 generation) and growth chamber (BC1S2 and BC1S3 generations), and identified several lines of agronomic interest. Introgression analysis was performed (BC1S3 generation) to assess the level of integration of C. oxyacanthus DNA into the C. tinctorius genome, and results are suggestive of less inter-specific recombination than expected.

• Subjects / Keywords

  • Linkage mapping
  • introgression
  • safflower
  • Carthamus tinctorius
  • Carthamus oxyacanthus
  • oilseed

• Graduation date

  Spring 2011

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3589K

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.