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Hypothermic and Subnormothermic Ex-situ Heart Perfusion: Examining Diverse Strategies and Their Effects on Heart Function Preservation
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- Author / Creator
- Mainardi Aguiar da Silva, Guilherme
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The number of people suffering from heart failure grows annually. For those people who have advanced heart failure and are eligible for a heart transplant, this is the gold standard procedure to treat them. Allowing not only an improvement in quality of life but also an increase in survival.
However, the number of heart transplants performed per year in Canada and around the world is smaller than the number of people on the waiting list for a donated organ. In addition, one of the determining factors for this deficit between donated organs and transplants performed is the current method of organ preservation.
Ex-situ heart perfusion (ESHP) has proven to be a more effective method than the current one, static cold storage (SCS). However, there are different types of ESHP, being the hypothermic and subnormothermic the least complex in relation to normothermic for several reasons that will be addressed in this thesis.
The creation of protocols to make ESHP uniform are essential for the dissemination of this method of organ preservation. Since this method can extend the preservation time prior to transplantation, it can improve preservation and hence increase the number of organs available for transplantation.
The first study presented evaluated the ideal temperature to be applied during hypothermic ESHP using UW machine perfusion solution (UWMPS). It was seen that the lowest temperature studied was the one that obtained better heart function preservation after 12 hours of perfusion. The results were compared to the current preservation method.
The second study sought to optimize UWMPS with the aim of enhancing heart function preservation. Two modifications were applied in the perfusate studied, the addition of amino acids and the addition of some ions (as these components are presented in Somah). The results obtained in this study were compared with UWMPS as a control.
The results presented in these two studies can be used to guide new studies and build new protocols for ESHP. This strengthens the use of ESHP as a gold standard method and increases the number of heart transplants performed. -
- Graduation date
- Fall 2024
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Library with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.