Characterization and silencing of differentially abundant proteins from Pyrenophora tritici-repentis

  • Author / Creator
    Fu, Heting
  • Tan spot, caused by Pyrenophora tritici-repentis, is an important foliar disease of wheat. Three genes (stp, unp and glu) encoding proteins previously found to be more abundant in pathogenic versus non-pathogenic fungal isolates were cloned, characterized, heterologously expressed, and silenced with the RNA silencing vector pSilent-1. The unp gene encoded a 16.9 kDa protein belonging to a superfamily of glycine-rich RNA-binding proteins, but growth and virulence of an unp-silenced strain of the fungus were not significantly different from the wild-type. The stp gene, which was not significantly silenced, encoded a 15.0 kDa protein homologous to a CipC-like antibiotic response protein. The glu gene encoded an exo-1,3-β-glucanase, 46.7 kDa in mass. Four glu-silenced strains were obtained. The strain in which silencing was strongest exhibited reduced growth, produced fewer appressoria, and caused less disease than the wild-type. The results suggest that exo-1,3-β-glucanase contributes to the development and virulence of P. tritici-repentis.

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  • Degree
    Master of Science
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