Insights Towards Optimizing Palladium-Catalyzed Bioorthogonal Reactions

  • Author / Creator
    Dubicki, Krystyn I
  • Despite large amounts of research investigating the mechanism and kinetics of the Suzuki-Miyaura reaction in organic solvent, very little research has been completed to investigate this mechanism or kinetic properties in aqueous solvent. Due to properties of the Suzuki-Miyaura reaction which are favourable to bioorthogonal reaction, I first investigated a method to study the kinetics of the Suzuki-Miyaura in water using NHC/Pd complexes that had been designed to facilitate cross-coupling in aqueous environments. I also designed a prototype assay for testing these catalysts that could be adapted for high-throughput screening. Synthesis towards a fluorescent probe for a high-throughput screen was also started. I determined that UV-Vis spectroscopy was not suitable for studying the Suzuki-Miyaura reaction in aqueous conditions, and that studying this reaction with fluorescent probes looks more promising.

  • Subjects / Keywords
  • Graduation date
    Fall 2021
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.