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Bacteriocins: Identification, structural elucidation, and mechanisms of resistance
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- Author / Creator
- Ibarra Romero, Cherry
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Bacteria employ various antimicrobial compounds to establish themselves within bacterial communities. Bacteriocins, which are ribosomally synthesized antimicrobial compounds, play a crucial role in this dynamic. This thesis focuses on strategies for purifying and identifying diverse bacteriocins and delves into the examination of resistance developed in Listeria monocytogenes strains against two distinct types of bacteriocins.
A strain called Enterococcus canintestini 49 (EC49), isolated from dog feces, displayed noteworthy antimicrobial activity against Clostridium perfringens, vancomycin-resistant enterococci, and Listeria monocytogenes. The research on EC49 centered on its bacteriocin production, revealing the identification of two bacteriocins through protein purification and analysis utilizing bacteriocin mining databases. Furthermore, the absence of genes related to virulence factors suggests the potential use of EC49 as a probiotic agent.
Antimicrobial resistance against leucocin A, a type IIa bacteriocin produced by Leuconostoc gelidum and against carnocyclin A, a circular bacteriocin produced by Carnobacterium maltaromaticum UAL 307, was identified in L. monocytogenes strains. The study aimed to determine if genetic changes in the mannose phosphotransferase system (Man-PTS), a docking molecule for type IIa bacteriocins, was responsible for the resistance. -
- Subjects / Keywords
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- Graduation date
- Spring 2024
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- Type of Item
- Thesis
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- Degree
- Doctor of Philosophy
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.