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Miniaturized genetic analysis systems based on microelectronic and microfluidic technologies

  • Author / Creator
    Behnam Dehkordi, Mohammad
  • Genetic analysis is not widely used for disease diagnostics as it is costly and very labour/infrastructure intensive. We believe that by employing both microelectronic and microfabrication technologies, we are able to integrate multiple functionalities into a single, manufacturable, inexpensive instrument that performs complete genetic analysis protocols. Cost reduction (i.e. instrument and reagent costs), smaller size, and higher automation (i.e. lower labour cost) will certainly pave the way for frequent use of genetic analysis for disease diagnostics. In this work, we develop technologies and techniques to implement a low power, inexpensive genetic analysis instrument that performs extraction of genetic material (e.g. DNA) from clinical samples (e.g. blood), genetic amplification (via polymerase chain reaction, PCR) and detection/analysis based on laser induced fluorescence (LIF)-capillary electrophoresis (CE), real-time PCR (rqPCR), and melting point analysis (MPA). This project involves integration of microfluidic and microelectronic technologies as well as molecular biology protocol adaptation. Furthermore, we develop technologies required to realize a single-use chip for genetic analysis. This chip, which is based on monolithic integration of microfluidics and microelectronics, can ultimately be mass produced using standard low-cost, high-volume microelectronic wafer fabrication equipment. We believe that the technologies developed here, along with the molecular biology protocol adaptations, will result in a low cost portable instrument that performs genetic analysis much faster, easier, and less expensive than conventional instruments. This will certainly revolutionize the use of genetic analysis for disease diagnostics.

  • Subjects / Keywords
  • Graduation date
    2010-11
  • Type of Item
    Thesis
  • Degree
    Doctor of Philosophy
  • DOI
    https://doi.org/10.7939/R33H7M
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Doctoral
  • Department
    • Department of Electrical and Computer Engineering
  • Supervisor / co-supervisor and their department(s)
    • Backhouse, Christopher (Electrical and Computer Engineering)
    • Elliott, Duncan (Electrical and Computer Engineering)
  • Examining committee members and their departments
    • Elliott, Duncan (Electrical and Computer Engineering)
    • Backhouse, Christopher (Electrical and Computer Engineering)
    • Gaudet, Vincent (Electrical and Computer Engineering)
    • Kostiuk, Larry (Mechanical Engineering)
    • Lapizco Encinas, Blanca (Microscale Bioseparations Laboratory, CINVESTAV-Monterrey, Mexico)