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Development of an optical biosensor based on Mach-Zehnder Interferometry for detection of Listeria monocytogenes

  • Author / Creator
    Sarkar, Dibyo
  • Label-free optical biosensors are widely used for the detection of pathogens due to their low-cost, real-time detection and high sensitivity. A Mach-Zehnder Interferometer (MZI) based biosensor has been developed for the detection of gram-positive bacteria, Listeria monocytogenes. An extensive theoretical analysis of MZI configurations was performed to determine the critical dimensions for a high sensitivity monomodal waveguide. These MZI waveguides, with nanometer size ridge structure, were fabricated using standard micro/ nanofabrication techniques. Using the fabricated MZI waveguides, an efficient optical immunoassay technique for the detection of Listeria was demonstrated. The MZI biosensor was able to detect Listeria at concentrations close to 10^5 cfu/ml, which is lower than the infection dose for human beings. SEM analysis and light intensity measurements showed the biosensor is highly selective to Listeria over other microbial species. Finally, a calibration scheme of the MZI biosensor was developed to determine an unknown concentration of Listeria in a given sample.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R35Q5J
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Mechanical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Mitra, Sushanta K. (Mechanical Engineering)
  • Examining committee members and their departments
    • Mitra, Sushanta K. (Mechanical Engineering)
    • Thundat, Thomas (Chemical and Materials Engineering)
    • Wang, Xihua (Electrical and Computer Engineering)