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Degradation mechanism of a Golgi-retained distal tubular acidosis mutant of the kidney Anion Exchanger 1 in renal cells

  • Author / Creator
    Berrini, Mattia
  • Distal renal tubular acidosis (dRTA) is a renal disease caused in some cases by mutations in the SLC4A1 gene encoding the kidney anion exchanger 1 (kAE1). Both recessive and dominant mutations result in mis-trafficking of proteins, preventing them from reaching the basolateral membrane of renal epithelial cells where their function is needed. kAE1 G701D is a functional, Golgi-retained dRTA mutant. The purpose of this thesis is to understand the degradation pathways of kAE1 G701D. We show that this mutant is poly-ubiquitylated and degraded by both lysosomal and proteasomal pathways. We provide evidence that the Nedd4 family interacting protein 1 (Ndfip1) interacts with kAE1 G701D, suggesting a possible role of Ndfip1 in the ubiquitylation process of this mutant. Also, we show that this mutant reaches temporarily the cell surface where it is endocytosed and degraded by the lysosomes via a peripheral quality-control machinery dependent mechanism. Furthermore we show that the function of kAE1 G701D is rescued at the cell surface upon inhibition of the lysosome and incubation with the chemical chaperone dimethyl sulfoxide (DMSO). This study suggests that modulating the peripheral quality-control machinery may provide novel therapeutic strategies for the treatment of dRTA patients.

  • Subjects / Keywords
  • Graduation date
    2014-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3V67B
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Physiology
  • Specialization
    • Cell Biology
  • Supervisor / co-supervisor and their department(s)
    • Cordat, Emmanuelle
  • Examining committee members and their departments
    • Young, James
    • Cordat, Emmanuelle
    • Leslie, Elaine
    • Casey, Joe