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Permanent link (DOI): https://doi.org/10.7939/R3T14TW55

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Autoregulation of RNA helicase operon expression Open Access

Descriptions

Other title
Subject/Keyword
CrhR
helicase
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Rosana, Albert Remus Romero
Supervisor and department
George W. Owttrim (Biological Sciences)
Examining committee member and department
Rebecca Case (Biological Sciences)
Michael Deyholos (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Microbiology and Biotechnology
Date accepted
2013-07-04T09:40:33Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The cyanobacterium Synechocystis sp. PCC 6803 encodes for an RNA helicase, crhR, whose expression is regulated by the redox status of the electron transport chain and further enhanced by temperature downshift. In this study, the effect of crhR inactivation was investigated in response to temperature alteration. The inactivation of CrhR has drastic morphological and physiological effects particularly under cold stress, including a rapid cessation of photosynthesis, impaired cell growth, decrease in viability, cell size and DNA content and accumulation of structural abnormalities. Extensive transcript and protein analyses between wild type and mutant cells revealed that CrhR activity is linked to its autoregulation of expression involving complex network acting at the transcriptional and post-transcriptional levels. Furthermore, autoregulation is extended to the rimO-crhR operon where processing and degradation contributes to differential accumulation of the cistrons. Lastly, the potential association of CrhR in a degradosome-polysome complex in the thylakoid membrane is proposed.
Language
English
DOI
doi:10.7939/R3T14TW55
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Rosana, A. R. R., Ventakesh, M., Chamot, D., Patterson-Fortin, L. M., Tarassova, O., Espie, G. S., & Owttrim, G. W. (2012). Inactivation of a low temperature-induced RNA helicase in Synechocystis sp. PCC 6803: physiological and morphological consequences. Plant and Cell Physiology, 53(4), 646-658.Rosana, A. R. R., Chamot, D., & Owttrim, G. W. (2012). Autoregulation of RNA Helicase Expression in Response to Temperature Stress in Synechocystis sp. PCC 6803. PloS one, 7(10), e48683.

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