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Permanent link (DOI): https://doi.org/10.7939/R37W9D

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Mitochondrial Dysfunction and Induction of Apoptosis in Hepatocellular Carcinoma and Cholangiocarcinoma Cell Lines by Thymoquinone Open Access

Descriptions

Other title
Subject/Keyword
Apoptosis
Hepatocellular carcinoma
Thymoquinone
Cholangiocarcinoma
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Abdualmjid,Reem J
Supervisor and department
Bamforth, Fiona (Co-supervisor, Department of Laboratory Medicine & Pathology)
Sergi, Consolato (Supervisor, Department of Laboratory Medicine & Pathology)
Examining committee member and department
Chan, Alicia (Department of Genetics)
Yap, Jason (Department of Pediatrics)
Department
Laboratory Medicine and Pathology
Specialization
Molecular Pathology
Date accepted
2014-12-10T15:59:07Z
Graduation date
2015-06
Degree
Master of Science
Degree level
Master's
Abstract
Thymoquinone, the plant-based bioactive constituent derived from the volatile oil of Nigella sativa, has been shown to possess considerable anti-neoplastic activity. The present study aimed to investigate the anti-tumour property of TQ against hepatocellular carcinoma (HepG2) and cholangiocarcinoma (HuCCT1) cells, the two most common primary hepatic tumours. All cell lines were treated with increasing concentrations of TQ for varying durations. The anti-proliferative effect of TQ was measured using the MTS assay and resulted in dose- and time-dependent growth inhibition in both cell lines. The analysis of cell cycle distribution, determination of apoptosis, assessment of morphological alterations and measurement of mitochondrial membrane potential changes were also investigated. TQ caused cell cycle arrest at different phases in the two cell types and induced apoptosis in both cell lines through the mitochondrial pathway. Overall, these findings suggest that TQ possesses promising therapeutic potential as an anti-tumour agent for treating hepatocellular carcinoma and cholangiocarcinoma.
Language
English
DOI
doi:10.7939/R37W9D
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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