Role of miRNA-126 in Hepatocellular Carcinoma and Cholangiocellular Carcinoma Open Access
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- Type of item
- Degree grantor
University of Alberta
- Author or creator
- Supervisor and department
Dr.Judith Hugh , Departmnet of Laboratory Medicine and Pathology
Dr. Consolato Sergi , Departmnet of Laboratory Medicine and Pathology
- Examining committee member and department
Dr. Xing-Zhen , Departmnet of Physiology
Dr.Fiona Bamforth , Departmnet of Laboratory Medicine and Pathology
Laboratory Medicine and Pathology
- Date accepted
- Graduation date
Master of Science
- Degree level
Hepatocellular carcinoma (HCC) and Cholangiocellular Carcinoma (CCA) represent the most common malignant tumor of the liver accounting about 90 % of all liver malignancies. The overall prognosis of HCC and CCA is very poor due to the lack of effective treatment.
MicroRNAs (miRNAs) represent a small endogenous non-coding RNAs that play a significant role in the regulation of gene expression post-transcriptionally. Altered expression of miRNAs has been observed in many malignancies including liver cancer. However, the expression level of miR-126 in HCC and CCA and its role in hepatic-carcinogenesis remains unclear. This thesis aimed to study the expression level, localization and biological significance of miRNA-126 in HCC and CCA.
In an effort to distinguish the expression pattern of miR-126 in HCC and CCA tissues and cell lines, two expression analysis has been used: in situ hybridization (ISH) and quantitative real time polymers chain reaction (QRT-PCR). Our ISH analysis has shown a significant reduction in miR-126 level in HCC and CCA tissues relative to their corresponding normal tissues. Moreover, an intensive expression of miR-126 in normal hepatocyte, blood vessels and sinusoid cells has been observed. Our qRT-PCR data demonstrated a lower expression level of miR-126 in HCC and CCA cell lines relative to a normal kidney cell line. By using several gain of functions analysis, this study demonstrated the effect of miR-126 in HCC and CCA cell lines. The over-expression of miR-126 in HepG2 and HuccT1 has significantly inhibited cell proliferation and growth. On the other hand, our data has shown that miR-126 overexpression inhibited cell ability to migrate.
Taken together, this study indicted that miR-126 could play a critical role in hepatic carcinogenesis. Indeed, miR-126 may serve as a novel suppressive miRNA in liver cancer. Furthermore, miR-126 may serve as potential therapy, diagnostic and prognostic biomarker.
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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