ERA

Download the full-sized PDF of Role of miRNA-126 in Hepatocellular Carcinoma and Cholangiocellular CarcinomaDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R35X25Q6V

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Role of miRNA-126 in Hepatocellular Carcinoma and Cholangiocellular Carcinoma Open Access

Descriptions

Other title
Subject/Keyword
miR-126
HCC
CCA
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Zailaie,Samar A
Supervisor and department
Dr.Judith Hugh , Departmnet of Laboratory Medicine and Pathology
Dr. Consolato Sergi , Departmnet of Laboratory Medicine and Pathology
Examining committee member and department
Dr. Xing-Zhen , Departmnet of Physiology
Dr.Fiona Bamforth , Departmnet of Laboratory Medicine and Pathology
Department
Laboratory Medicine and Pathology
Specialization
Molecular Pathology
Date accepted
2015-05-05T08:53:09Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
Hepatocellular carcinoma (HCC) and Cholangiocellular Carcinoma (CCA) represent the most common malignant tumor of the liver accounting about 90 % of all liver malignancies. The overall prognosis of HCC and CCA is very poor due to the lack of effective treatment. MicroRNAs (miRNAs) represent a small endogenous non-coding RNAs that play a significant role in the regulation of gene expression post-transcriptionally. Altered expression of miRNAs has been observed in many malignancies including liver cancer. However, the expression level of miR-126 in HCC and CCA and its role in hepatic-carcinogenesis remains unclear. This thesis aimed to study the expression level, localization and biological significance of miRNA-126 in HCC and CCA. In an effort to distinguish the expression pattern of miR-126 in HCC and CCA tissues and cell lines, two expression analysis has been used: in situ hybridization (ISH) and quantitative real time polymers chain reaction (QRT-PCR). Our ISH analysis has shown a significant reduction in miR-126 level in HCC and CCA tissues relative to their corresponding normal tissues. Moreover, an intensive expression of miR-126 in normal hepatocyte, blood vessels and sinusoid cells has been observed. Our qRT-PCR data demonstrated a lower expression level of miR-126 in HCC and CCA cell lines relative to a normal kidney cell line. By using several gain of functions analysis, this study demonstrated the effect of miR-126 in HCC and CCA cell lines. The over-expression of miR-126 in HepG2 and HuccT1 has significantly inhibited cell proliferation and growth. On the other hand, our data has shown that miR-126 overexpression inhibited cell ability to migrate. Taken together, this study indicted that miR-126 could play a critical role in hepatic carcinogenesis. Indeed, miR-126 may serve as a novel suppressive miRNA in liver cancer. Furthermore, miR-126 may serve as potential therapy, diagnostic and prognostic biomarker.
Language
English
DOI
doi:10.7939/R35X25Q6V
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2015-05-05T14:53:09.950+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 2436872
Last modified: 2016:06:24 17:02:07-06:00
Filename: Zailaie_Samar_A_201504_MSc.pdf
Original checksum: deaf9a725bd87582b801088dd80ece03
Activity of users you follow
User Activity Date