Download the full-sized PDF of Influence of lipids (arachidonic acid and cholesterol) on calcium signalling in rodent pancreatic beta cellsDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

Influence of lipids (arachidonic acid and cholesterol) on calcium signalling in rodent pancreatic beta cells Open Access


Other title
pancreatic beta cells
fluorescent calcium imaging
intracellular calcium concentration
arachidonic acid
extracellular calcium entry
intracellular calcium release
Type of item
Degree grantor
University of Alberta
Author or creator
Yeung-Yam-Wah, Valerie
Supervisor and department
Tse, Amy (Centre for Neuroscience and Department of Pharmacology)
Examining committee member and department
Colmers, William F. (Centre for Neuroscience and Department of Pharmacology)
Chaves, Elena Posse de (Department of Pharmacology)
Braun, Andrew P. (Department of Physiology and Pharmacology, University of Calgary)
Karpinski, Edward (Department of Physiology)
Centre for Neuroscience

Date accepted
Graduation date
Doctor of Philosophy
Degree level
Ca2+ is an important mediator of stimulus-secretion coupling in beta cells of the pancreatic islets, which secrete insulin in response to elevation in plasma glucose concentration. I studied the actions of two lipids, arachidonic acid (AA) and cholesterol, on enzymatically-dissociated single beta cells of rat and mouse, using cytosolic Ca2+ ([Ca2+]i) measurement in conjunction with whole-cell patch-clamp techniques. AA, which is produced in the beta cell upon stimulation with either glucose or acetylcholine, was found to induce a large increase in [Ca2+]i that was dependent on both extracellular Ca2+ entry and intracellular Ca2+ release. Part of the AA-mediated extracellular Ca2+ entry was due to Ca2+ influx through the arachidonate-regulated Ca2+ (ARC) channels, which have not previously been reported in beta cells. The AA-mediated intracellular Ca2+ release was a result of Ca2+ mobilization from multiple inositol trisphosphate (IP3)-sensitive intracellular stores, including the endoplasmic reticulum (ER) and an acidic Ca2+ store that is probably the secretory granules. Therefore, in beta cells, the AA-mediated Ca2+ signal may amplify the [Ca2+]i rise induced by insulin secretagogues. Cholesterol is an integral component of cellular membranes and an important regulator of cellular functions. However, elevation of cholesterol level in the pancreatic islets reduces glucose-stimulated insulin secretion. I found that cholesterol overload impairs the glucose-stimulated [Ca2+]i increase in beta cells by two major mechanisms: the first is a decrease in glucose-stimulated ATP production, which is partly mediated by a decrease in glucose uptake, and the second is the reduction of voltage-gated Ca2+ current density. These effects of cholesterol may partly account for the decreased insulin secretion that develops in patients with type II diabetes, who typically exhibit hypercholesterolemia. In summary, different lipids may mediate beneficial or detrimental effects on Ca2+ regulation in rodent pancreatic beta cells.
License granted by VALERIE YEUNG-YAM-WAH ( on 2010-08-09T16:47:14Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1041155
Last modified: 2015:10:12 18:04:01-06:00
Filename: Yeung-Yam-Wah_Valerie_Fall2010.pdf
Original checksum: a913775d7959c607661c17aaa578ba1d
Well formed: true
Valid: true
File title: Microsoft Word - Yeung-Yam-Wah_Valerie_Fall2010.doc
File author: p3ad
Page count: 149
Activity of users you follow
User Activity Date