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Investigation of osteogenic and adhesive properties of Rosette nanotubes Open Access


Other title
Type of item
Degree grantor
University of Alberta
Author or creator
Agrawal, Rahul
Supervisor and department
Fenniri, Hicham (Chemistry), Supervisor
Examining committee member and department
Jomha, Nadr (Surgery), Committee member
Burrell, Robert (Biomedical Engineering), Committee Chair
Uludag, Hasan (Chemical and Materials engineering), co-Supervisor
Department of Biomedical Engineering
Biomedical Sciences
Date accepted
Graduation date
Master of Science
Degree level
This thesis is focussed on bone tissue engineering for improving the osseointegration of joint implants. We have incorporated nano-scaled materials called as Rosette Nanotubes (RNTs) which mimic the geometry of the natural components of bone with the growth factor, Bone Morphogenetic Protein-7 (BMP-7). In particular, short peptide sequences were identified from BMP-7 (SNVILKKYRN, KPSSAPTQLN, and KAISVLYFDDS) and were linked with Twin K1 RNTs. The first stage of our study was focused on the purification and characterization of these short peptides co-assembled with RNTs using HPLC (High Performance Liquid Chromatography) and their characterization by SEM (Scanning Electron Microscopy) respectively. The Second stage involved cyto-compatibility studies of the RNTs. The third stage involved the adhesion studies of RNTs coated on different substrates. The fourth and final stage was the osteogenic differentiation studies of these materials. Results have shown that the HPLC purification yield was 12-20% and SEM characterization showed the presence of successfully assembled nanotubes. Coating of RNTs on different substrates has shown a significant increase in adhesion of human Bone Marrow Stromal Cells (hBMSCs) on tissue culture treated plates and non-tissue culture treated plates. Moreover, peptide co-assemblies with Twin K1 RNTs have shown a significant increase in specific ALP (Alkaline phosphatase) activity. In summary, Twin K1 RNTs and peptide co-assembled RNTs have shown promising results for improving the cell adhesion on plastic substrates (treated and non-treated plates). Moreover, peptide co-assembled RNTs have shown early signs of osteogenic differentiation.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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