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Permanent link (DOI): https://doi.org/10.7939/R3ZS2KK97

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Detection of Foodborne Pathogenic Bacteria using Bacteriophage Tail Spike Proteins Open Access

Descriptions

Other title
N/A
Subject/Keyword
Phage Tail Spike Proteins
PCR
Bacterial Detection
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Poshtiban, Somayyeh
Supervisor and department
Evoy, Stephane (Department of Electrical and Computer Engineering)
Examining committee member and department
Zemp, Roger (Department of Electrical and Computer Engineering)
Turner, Robin (The University of British Columbia)
Barlage, Doug (Department of Electrical and Computer Engineering)
Sameoto, Dan (Department of Mechanical Engineering)
Jiang, Hai (Department of Electrical and Computer Engineering)
Department
Department of Electrical and Computer Engineering
Specialization
Micro-Electromechanical Systems and Nanosystems
Date accepted
2014-01-28T13:19:58Z
Graduation date
2014-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Foodborne infections are worldwide health problem with tremendous social and financial impacts. Efforts are focused on developing accurate and reliable technologies for detection of food contaminations in early stages preferably on-site. This thesis focuses on interfacing engineering and biology by combining phage receptor binding proteins (RBPs) with engineered platforms including microresonator-based biosensors, magnetic particles and polymerase chain reaction (PCR) to develop bacterial detection sensors. We used phage RBPs as target specific bioreceptors to develop an enhanced microresonator array for bacterial detection. These resonator beams are optimized to feature a high natural frequency while offer large surface area for capture of bacteria. Theoretical analysis indicates a high mass sensitivity with a threshold for the detection of a single bacterial cell. We used phage RBPs as target specific bioreceptors, and successfully demonstrated the application of these phage RBB-immobilized arrays for specific detection of C. jejuni cells. We also developed a RBP-derivatized magnetic pre-enrichment method as an upstream sample preparation method to improve sensitivity and specificity of PCR for detection of bacterial cells in various food samples. The combination of RBP-based magnetic separation and real-time PCR allowed the detection of small number of bacteria in artificially contaminated food samples without any need for time consuming pre-enrichment step through culturing. We also looked into integration of the RBP-based magnetic separation with PCR onto a single microfluidic lab-on-a-chip to reduce the overall turnaround time.
Language
English
DOI
doi:10.7939/R3ZS2KK97
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
S. Poshtiban, M. A. Javed, D. Arutyunov, A. Singh, G. Banting, C. M. Szymanski and S. Evoy, Phage receptor binding protein-based magnetic enrichment method as an aid for real time PCR detection of foodborne bacteria, Analyst, 2013, 138, 5619 - 5626

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