ERA

Download the full-sized PDF of The poloxamer 407 induced hyperlipidemic rat model and its effect on renal toxicity of calcineurin inhibitorsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3KX5B

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

The poloxamer 407 induced hyperlipidemic rat model and its effect on renal toxicity of calcineurin inhibitors Open Access

Descriptions

Other title
Subject/Keyword
Hyperlipidemia, Poloxamer 407, Cyclosporine, Tacrolimus
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Chaudhary, Hetal R
Supervisor and department
Brocks, Dion (Pharmacy and Pharmaceutical Sciences)
Examining committee member and department
El-Kadi, Ayman (Pharmacy and Pharmaceutical Sciences)
Brocks, Dion (Pharmacy and Pharmaceutical Sciences)
Baker, Glen (Psychiatry and Centre for Neuroscience)
Seubert, John (Pharmacy and Pharmaceutical Sciences)
Department
Faculty of Pharmacy and Pharmaceutical Sciences
Specialization
Pharmaceutical Sciences
Date accepted
2012-09-28T11:11:13Z
Graduation date
2012-09
Degree
Master of Science
Degree level
Master's
Abstract
The present study characterized poloxamer 407 (P407) induced hyperlipidemia in rats and investigated its effect of on renal toxicity of the immunosuppressants tacrolimus and cyclosporine A. P407 (0.5 or 1 g/kg) was injected in rats and blood samples were collected at different time-points. Serum levels of total cholesterol, triglyceride, high-density lipoprotein (HDL), adiponectin, leptin and TNF-α were measured. In vitro renal toxicity studies were performed using LLC-PK1 and NRK-52E cells for tacrolimus and cyclosporine A, respectively. P407 increased serum cholesterol, triglyceride and HDL with maximum increase at 36h. Moreover, P407 significantly increased leptin and decreased adiponectin but did not affect TNF-α. Hyperlipidemic serum treated cells did not show any significant difference in toxicity compared to normo-lipidemic serum treated cells for both the drugs. Together, P407 induces hyperlipidemia and alters lipid and adipokine levels. Furthermore, hyperlipidemic serum treatment had no apparent effect on in vitro kidney cell toxicity by immunosuppressants.
Language
English
DOI
doi:10.7939/R3KX5B
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-05-01T00:08:22.149+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1348964
Last modified: 2015:10:12 17:34:39-06:00
Filename: Hetal thesis printing correction 27 sep.pdf
Original checksum: 02251925e7a71a3b53e7549007f506b2
Well formed: true
Valid: true
File author: Hetal
Page count: 102
File language: en-CA
Activity of users you follow
User Activity Date