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The impact of various ozone pretreatment doses on the performance of endogenous microbial communities for the remediation of oils sands process-affected water Open Access


Other title
Naphthenic acids
Ozonated OSPW
Type of item
Degree grantor
University of Alberta
Author or creator
Dong, Tao
Supervisor and department
Dr. Yang Liu and Dr. Gamal EI-Din
Examining committee member and department
Dr. Gamal EI-Din (Environmental Engineering)
Dr. Yaman Boluk (Materials Engineering)
Dr. Yang Liu (Environmental Engineering)
Department of Civil and Environmental Engineering
Environmental Engineering
Date accepted
Graduation date
Master of Science
Degree level
In this study, the effects of different ozone pretreatment doses on the performance of endogenous microbial populations in degrading naphthenic acids (Nash and Traver) for the treatment of oil sands process-affected water (OSPW) were evaluated. The results showed that ozonation enhanced the biodegradability of OSPW and the maximum COD removal through biodegradation which occurred at utilized ozone dose of 50 mg/L. After pretreatment with the highest utilized ozone dose of 200 mg/L and bioreactor operation for 73 days, the batch bioreactor removed more than 80% of the chemical oxygen demand (COD), it also removed greater than 95% of the acid extractable fraction (AEF) from the OSPW. High-resolution mass spectrometry analysis showed complete degradation of NAs with specific degrees of cyclization (Z= -2 and -4) after combined treatment of ozonation and biodegradation. Furthermore, with increasing the utilized ozone, the total bacterial number increased while specific bacterial strains such as Sphingomonadaceaeand Azoarcus have shown to improve the bioreactor performance. At high pretreatment utilized doses of ozone (116 and 200 mg/L), the biodegradation completely eliminated the acute toxicity of OSPW towards Vibrio fischeri (IC20> 100% v/v). In summary, increasing the ozone dose used for pretreatment of OSPW showed clear benefits regarding the removal of organic compounds, the growth of bacteria, and the reduction of toxicity.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Hwang, G., T. Dong, M. S. Islam, Z. Sheng, L. A. Pérez-Estrada, Y. Liu and M. Gamal El-Din (2013). "The impacts of ozonation on oil sands process-affected water biodegradability and biofilm formation characteristics in bioreactors." Bioresour. Technol. 130: 269-277.

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