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Permanent link (DOI): https://doi.org/10.7939/R3PG7J

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Proteomic analysis of wheat (Triticum aestivum) whole roots and cell walls under water-deficit stress Open Access

Descriptions

Other title
Subject/Keyword
Water-deficit
Plant
Stress
Proteomics
Wheat
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ganesh, Shiv
Supervisor and department
Kav, Nat (Agriculture, Food and Nutritional Sciences)
Deyholos, Michael (Biological Sciences)
Examining committee member and department
Good, Allen (Biological Sciences)
Department
Department of Biological Sciences
Specialization

Date accepted
2011-01-30T02:46:49Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
Wheat plants are affected by water-deficit stress in various regions of the world resulting in reduced crop productivity and thus decreased food production. To better understand the protein changes of water-deficit stress in wheat roots, comparative proteomics was performed using 2D gel electrophoresis followed by HPLC-MS/MS. Forty proteins were identified, twenty-nine of which are non-redundant. Cell walls root proteins were identified using SDS-PAGE followed by mass spectrometry, resulting in the identification of seventeen proteins. Cell wall polysaccharides were extracted from roots to identify the polysaccharide metabolic changes that occurred under water-deficit stress conditions. Using FT-IR, cellulose was found to increase while hemicellulose and pectin content decreased. Further analysis by PCA showed changes in overall polysaccharide content over time. Overall, proteins identified in wheat whole roots and cell walls, combined with indications of polysaccharide modifications in the root cell walls, give us a better understanding of wheat responses to water-deficit stress.
Language
English
DOI
doi:10.7939/R3PG7J
Rights
License granted by Shiv Ganesh (shiv@ualberta.ca) on 2011-01-28T02:43:09Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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