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Permanent link (DOI): https://doi.org/10.7939/R34F1MS6M

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The Effects of Docosahexaenoic Acid (DHA) Dietary Supplementation in a Mouse Model of Stargardt-like Macular Dystrophy (STGD3) Open Access

Descriptions

Other title
Subject/Keyword
Docasahexaenoic Acid (DHA)
Animal Model
Retinal Degeneration
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Hong, Mandy
Supervisor and department
Sauvé, Yves (Ophthalmology)
Examining committee member and department
Field, Catherine (Human Nutrition)
Allison, Ted (Biological Sciences)
Chan, Catherine (Human Nutrition)
Department
Centre for Neuroscience
Specialization

Date accepted
2013-07-09T11:10:59Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Underlying mechanisms of how docosahexaenoic acid (DHA) might impact the progression of macular degeneration are unknown. We relied on the ELOVL4 mouse model of juvenile macular degeneration, STGD3, to test the hypothesis that antenatal DHA dietary supplementation (2% w/w of total fatty acid) would slow down the progression of retinal degeneration as evidenced by preserved function, anatomy, and DHA levels. DHA+, DHA-, and chow diets commenced antenatally. Retina function was assessed by electroretinogram at 1 and 3 months, while anatomical integrity and fatty acid profiles were assessed with cross-sectional staining and UPLC/MS/MS, respectively, at 3 months. Results showed a negative ELOVL4 genotype effect on rod function, photoreceptor numbers, and DHA/AA levels. Surprisingly, in transgenic retinas DHA supplementation resulted in lower DHA levels compared to non-supplemented. Although beneficial effects of DHA have been reported, antenatal and 3 month postnatal supplementation is not sufficient to elicit these effects in mice with STGD3.
Language
English
DOI
doi:10.7939/R34F1MS6M
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File size: 2120322
Last modified: 2015:10:12 15:46:47-06:00
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File title: University of Alberta
File author: Sauve Lab
Page count: 117
File language: en-CA
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