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Hyperbranched Phosphorylcholine Polymers Synthesized via RAFT Polymerization for Gene Delivery and Synthesis of an Elastomeric Conductive Polymer for Cardiovascular Applications Open Access


Other title
gene delivery
Type of item
Degree grantor
University of Alberta
Author or creator
Jawanda,Manraj S
Supervisor and department
Narain, Ravin (Chemical Engineering)
Examining committee member and department
Zeng, Hongbo (Chemical Engineering)
Liu, Yang (Envrionmental Engineering)
Gupta, Rajender (Chemical Engineering)
Department of Chemical and Materials Engineering
Process Control
Date accepted
Graduation date
Master of Science
Degree level
Gene therapy has the potential to treat a variety of hereditary diseases such as diabetes, peanut anaphylaxis, cystic fibrosis and different types of cancer. Gene therapy relies on the design of a delivery system which can carry a gene of interest into a cell. The delivery systems can be broken down into two different types: viral and non-viral carriers. Viruses were the standard for gene delivery and showed high cell transfection, but on the other hand would sometimes induce an immunological response. As a result, there has been a growing interest towards non-viral carriers such as polymers which are less toxic and biocompatible. Polyethyleneimine (PEI) is the current standard for non-viral gene carriers, but has drawbacks such as high toxicity. The effect of molecular weight and composition on transfection efficiency has been evaluated for many polymers, but very few studies have been done on polymer architecture. This study focused on the synthesis of a hyperbranched phosphorylcholine cationic copolymer that incorporates a nontoxic and biocompatible polymer known as poly(2-Methacryoyloxyethyl Phosphorylcholine (MPC)). The hyperbranched copolymers were synthesized via RAFT polymerization through the incorporation of a cross linking agent and showed broad molecular weight distributions. Complete binding to DNA was achieved with the cationic polymers, however the gene expression was found to be significantly lower than PEI when transfected into Hep G2 cells. In the second part of this thesis, elastomer films were embedded with conductive materials for potential cardiovascular applications. Polydimethylsiloxane (PDMS) is an elastomer used in a variety of medical applications because of its biocompatibility. In this study, Poly(p-phenylene vinylene) (PPV) and Multi-Walled Carbon Nanotubes (MWNT) which were two different conductive materials, were embedded into PDMS and composite films of PPV/PDMS and MWNT/PDMS were synthesized.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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