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The Proteome of Partial Bladder Outlet Obstruction & The Therapeutic Role of Mesenchymal Stem Cells Open Access


Other title
Partial Bladder Outlet Obstruction
Mesenchymal Stem Cells
Type of item
Degree grantor
University of Alberta
Author or creator
Alsaikhan, Bader H
Supervisor and department
Metcalfe, Peter D. (Surgery)
Examining committee member and department
Adesida, Adetola (Surgery)
Ding, Jie (Surgery)
Churchill, Thomas A (Surgery)
Department of Surgery
Experimental Surgery
Date accepted
Graduation date
Master of Science
Degree level
Introduction: Partial bladder outlet obstruction (pBOO) is a common problem in urology. It passes through several pathological phases before progressing to bladder fibrosis. Herein, we investigated the proteomic profile of the urinary bladder in the early phase after pBOO, and examined the therapeutic role of mesenchymal stem cells (MSCs) in this pathology. Methods: Proteomic analysis of bladder tissue was performed using liquid- chromatography – mass spectrometry. The effects of intravenous injection of MSCs were examined on bladder function and gene transcription of previously described factors and newly detected proteins after proteomic analysis. Results: pBOO induced an increase in inflammatory, hypoxic, and cellular stress markers. Treatment with MSCs improved these molecular changes and helped maintain normal bladder capacity. Conclusion: Pathological changes happen early after pBOO. Inflammation, hypoxia, and mechanical strain seem to be the main factors in initiating the pathological cascade. Treatment with MSCs was successful in halting the inflammatory and hypoxic changes in the early phase after pBOO.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Abstract: Early Changes in Proteomic Profile of Urinary Bladder After Partial Bladder Outlet Obstruction. Bader H. Alsaikhan*, Jie Ding, Edward E. Tredget, Richard Fahlman, Peter D. Metcalfe, Edmonton, Canada. Link:

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