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Soluble negative regulators of goldfish primary kidney macrophage development Open Access


Other title
Soluble negative regulators of macrophage development
Goldfish PKM
Primary kidney macrophage
Type of item
Degree grantor
University of Alberta
Author or creator
Nono, Berhanu
Supervisor and department
Dr. Daniel Barreda ( Biological Sciences)
Examining committee member and department
Dr. James Stafford (Biological Sciences)
Dr. Leluo Guan (Agricultural, Food & Nutritional Sciences)
Dr. Mike Belosevic (Biological Sciences)
Department of Biological Sciences

Date accepted
Graduation date
Master of Science
Degree level
The generation of macrophages requires the coordinated responses to stimulatory and inhibitory signals that cell receive from their environment. While the up-regulation of macrophage production and survival is essential to fortify the immune system, their down-regulation is also vital to prevent macrophage related diseases and malignancy. Previous studies in goldfish showed that primary kidney macrophages release endogenous growth inducing factors into proliferative phase supernatant, which up-regulate their proliferation and survival. In this thesis, the effect of senescence phase supernatant (collected from goldfish primary kidney macrophage cultures) on goldfish primary kidney macrophages proliferation, survival and its impact on the ability of macrophages to tolerate H2O2 was analyzed. The results showed that the senescence phase supernatant down-regulated the proliferation and survival, and decreased the chemical tolerance of the cells. This indicated that the goldfish primary kidney macrophages promote targeted control of their proliferation and survival by secreting endogenous growth inhibitory factors in the senescence phase supernatant.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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