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Semiochemical-Mediated Interactions of an Invasive Leaf miner, Caloptilia fraxinella (Lepidoptera: Gracillariidae), on a Non-Native Host, Fraxinus spp. (Oleaceae) and its Native Parasitoid, Apanteles polychrosidis (Hymenoptera: Braconidae) Open Access


Other title
antennal sensilla
ash leaf coneroller
host location
Type of item
Degree grantor
University of Alberta
Author or creator
Wist, Tyler J
Supervisor and department
Dr. Maya Evenden
Examining committee member and department
Dr. Nadir Erbilgin, Department of Renewable Resources
Dr. B. Staffan Lindgren, University of Northern British Columbia
Dr. Jessamyn Manson, Department of Biological Sciences
Dr. J.C. Cahill, Department of Biological Sciences
Department of Biological Sciences
Date accepted
Graduation date
Doctor of Philosophy
Degree level
The ash leaf-coneroller, Caloptilia fraxinella (Ely) (Lepidoptera: Gracillariidae) is an introduced moth to the urban forest of Western Canada that specializes on ash (Fraxinus spp.) (Oleaceae). It uses green ash, F. pensylvannica Marsh. var. subintegerrima (Vahl) Fern, and black ash, F. nigra Marsh in its expanded range but prefers black over green ash for oviposition. The oviposition preference for black ash was not linked to enhanced performance of offspring on black ash as green ash supported higher larval survival and faster development than black ash. Younger leaflets are preferred by female moths over older leaflets and green ash elicits more host location flight by gravid females than black ash in wind tunnel experiments. Caloptilia fraxinella adults are attracted to volatile organic compounds (VOCs) released from ash seedlings. The antennae of mated female C. fraxinella consistently detect six VOCs released from black ash and green ash, four of which are common to both species. Synthetic copies of these VOCs elicited as much oriented upwind flight in mated female C. fraxinella as green and black ash seedlings but did not elicit contact with the VOC lure. Lures do not attract more female C. fraxinella than unbaited control traps in field experiments. These experiments lay the foundation for further development of semiochemical lures based on host VOCs to monitor and potentially control populations of C. fraxinella. Apanteles polychrosidis Vierek (Hymenoptera: Braconidae), is identified here as the dominant parasitoid of C. fraxinella in its expanded range. It exhibits protandrous adult emergence and adults mate soon after females emerge. The remainder of the parasitoid complex affecting C. fraxinella in Edmonton is, in order of parasitism rate, Diadegma Förster (near D. fenestrale (Holmgren)) (Hymenoptera: Ichneumonidae), three Sympiesis Förster species (Hymenoptera: Eulophidae) (S. sericeicornis (Nees von Esenbeck), one near S. viridula (Thomson) and one unknown Sympiesis species, and Pteromalus phycidis (Ashmead) (Hymenoptera: Pteromalidae). Of these parasitoids, only A. polychrosidis is present in numbers high enough to control populations of C. fraxinella. Parasitism rate by A. polychrosidis of C. fraxinella on black ash is higher than on green. Parasitism is positively dependent on C. fraxinella density on black ash but is negatively density dependent on green ash. Apanteles polychrosidis exhibits differential attraction to the VOCs of each ash species which may mediate the differential parasitism response. In olfactometer experiments, A. polychrosidis use host location cues induced by feeding damage on black ash but not on green ash. Thirteen ash VOCs are detected by the antennae of A. polychrosidis.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Wist TJ, Evenden ML (2013) Parasitoid complex and bionomics of Apanteles polychrosidis (Hymenoptera: Braconidae) on the ash leaf-cone roller (Lepidoptera: Gracillariidae). Can Entomol 145:416-429.

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