Forward and Side Two Dimensional Scattered Light Patterns Studies of Single Cell for Label-Free Flow Cytometry

• Author / Creator

  Shahin, Hesam

• A technique using two dimensional scattered light patterns from single cells to extract useful information from cells has been investigated experimentally and numerically. The technique can be implemented in label-free cytometry as an alternative to conventional fluorescent-activated flow cytometry. In this thesis two dimensional scattered light patterns in both forward and side directions from latex beads with spherical and elliptical shapes, Hematopoietic Stem Cells and Very Small Embryonic Like Stem Cells have been studied. The ability to measure the patterns of a cell in forward and side direction simultaneously is an important step forward for such technique. In particular it has been demonstrated that useful information of a cell under investigation including the size and shape of its nucleus and its mitochondria distribution can be obtained from its simultaneously captured forward and side two dimensional scattered light patterns.

• Subjects / Keywords

  • Light scattering study
  • VSEL
  • Label-free
  • Flow cytometry

• Graduation date

  Spring 2016

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R30P0X346

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.