ERA

Download the full-sized PDF of Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate InteractionsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3610W59D

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions Open Access

Descriptions

Other title
Subject/Keyword
galectin
desorption electrospray ionization
library screening
lectin
protein-carbohydrate interactions
human milk oligosaccharides
mass spectrometry
electrospray ionization
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Shams-Ud-Doha, Km
Supervisor and department
Klassen, John (Department of Chemistry)
Examining committee member and department
Brown, Alexander (Department of Chemistry)
Le, Chris (Department of Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2016-06-30T09:29:33Z
Graduation date
2016-06:Fall 2016
Degree
Master of Science
Degree level
Master's
Abstract
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer solutions. Protein-carbohydrate interactions were quantified using liquid sample DESI-MS and it was found to be in agreement with values obtained by the direct ESI-MS assay. A rapid and quantitative method was developed to screen oligosaccharides libraries against lectins by the proxy protein ESI-MS assay. In this present work, the proxy protein ESI-MS method combined with direct ESI-MS protein-ligand binding assay and competitive protein binding assay was developed to detect and quantify interactions of protein target e.g. pathogenic protein, with glycan library. When direct detection of target protein by ESI-MS is not possible this method will facilitate the quantification of protein against ligand libraries. This method was applied to screen small oligosaccharide libraries against the N-terminal fragment of the family 51 carbohydrate-binding module, a fucose-binding bacterial lectin from Ralstonia solanacearum and the P particle of human norovirus, to demonstrate the reliability and versatility of the assay. Finally, comparative studies of human milk oligosaccharides (HMOs) specificities of human galectin-1, 3, 9 were described by using direct ESI-MS assay as compared to glycan array method. Human milk oligosaccharides (HMOs) have a variety of biological functions against pathogens and serve as prebiotic. To quantify the interaction between three human galectins and 32 HMOs, electrospray ionization mass spectrometry (ESI-MS) direct binding assay method was used. The data show that galectins have broad HMO specificity with association constants ranging from 103 to 105 M-1 along with no affinity for several specific HMOs. Binding affinity of galectins was found to be similar to binding affinity to histo-blood type oligosaccharides. Weak or no binding was established for galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS). These values were compared to values obtained from glycan array analysis. Significant difference was observed which depicts the reliability of the direct ESI-MS assay for quantifying protein-ligand interaction.
Language
English
DOI
doi:10.7939/R3610W59D
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
Yao, Y., Shams-Ud-Doha, K., Daneshfar, R., Kitova, E. N., Klassen, J. S., J. Am. Soc. Mass Spectrom., 2015, 26, 98-106.Shams-Ud-Doha, K. (equal contribution), Han, L. (equal contribution), Kitova, E. N., Klassen, J. S., Anal. Chem., (revision requested and resubmitted).

File Details

Date Uploaded
Date Modified
2016-06-30T15:29:34.895+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 9284501
Last modified: 2017:11:08 16:26:29-07:00
Filename: Shams-Ud-Doha_Km_201606_MSc.pdf
Original checksum: 0ecf04a5731530200c76bc99345a9ffd
Activity of users you follow
User Activity Date