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Permanent link (DOI): https://doi.org/10.7939/R3PM08

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Genetic analysis of the B-genome chromosomes in the Brassica species Open Access

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Other title
Subject/Keyword
Genetics, brassica species, introgression
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Navabi, Zahra
Supervisor and department
Dr. Habibur Rahman, Agricultural, Food and Nutritional Science
Dr. Allen Good, Biological Sciences
Examining committee member and department
Dr.Dean Spaner, Agricultural, Food and Nutritional Science
Dr. Rachel Scarth, University of Victoria
Dr. Francis Yeh, Renewable resources
Dr. Stephen Strelkov, Agricultural, Food and Nutritional Science
Department
Department of Agricultural, Food and Nutritional Science
Specialization

Date accepted
2009-10-15T21:35:40Z
Graduation date
2009-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
The family Brassicaceae includes a number of important species used as vegetables, oilseeds and medicine. The Brassica B-genome is significantly diverged from the A- and C-genomes, and species containing the B-genome possess many valuable agronomic and disease resistance traits. In this thesis, two populations of interspecific crosses between Brassica napus (AACC) and Brassica carinata (BBCC) were studied, and microsatellite (SSR) markers and genomic in situ hybridization (GISH) techniques were applied to characterize the B-genome chromosome introgressions in the advanced backcross populations and their effect on disease resistance, morphological and seed quality traits. A BC2S3 derived doubled haploid (DH) population was genotyped with 184 SSR markers and DH lines carrying stable B-genome chromosomal segments were identified. The GISH assay demonstrated that three of the 60 DHs were substitution lines in which the B-genome chromosome J13 was common to all. The lines with B-genome chromosomes, “B+”, were significantly different (P <0.01) from the lines without B-genome chromosomes, “B-” for some morphological and seed quality traits, such as days to flowering, days to maturity and erucic acid content. Further study revealed that resistance to Sclerotinia stem rot (caused by Sclerotinia sclerotiorum) has been inherited from the B or C genomes of B. carinata in some of the DH lines. Several BC3S1 families of another cross, derived from well-characterized BC3 plants containing introgressed B-genome chromosomes, were analyzed using SSR markers and the GISH assay to study the inheritance of the B-genome chromosome(s) and their association with morphological traits. This study revealed that the B-genome chromosomes tend to transfer through generations, either as whole chromosome(s) or as chromosomes without small terminal segments, as evidenced by low recombination frequencies (~ 0%) between the B and A/C-genome chromosomes. These results were supported by the GISH assay and chromosomes counts, and revealed that many of the BC3S1 lines were addition lines carrying extra B-genome chromosomes.
Language
English
DOI
doi:10.7939/R3PM08
Rights
License granted by Zahra Navbai (znavabi@ualberta.ca) on 2009-10-09T19:28:50Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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