Download the full-sized PDF
This file is in the following communities:
|Department of Biological Sciences|
This file is in the following collections:
|Journal Articles (Biological Sciences)|
Primary Structure and Function of Three Gonadotropin- Open Access
- Author or creator
- Additional contributors
- Type of item
- Journal Article (Published)
The evolution of GnRH and the role of multiple forms within the brain are examined. Three forms of GnRH were purified from the brain of Pacific herring (Clupea harengus pallasi) and characterized using Edman degradation and mass spectrometry. Two forms correspond with the known structures of chicken GnRH-II and salmon GnRH that are found in many vertebrate species. The third form, designated herring GnRH (hrGnRH), has a primary structure of pGlu-His-Trp-Ser-His-Gly-Leu-Ser-Pro-Gly-NH2. This novel peptide is a potent stimulator of gonadotropin II and GH release from dispersed fish pituitary cells. The content of hrGnRH in the pituitary was 8-fold that of salmon GnRH and 43-fold that of chicken GnRH-II, which provides supporting evidence that hrGnRH is involved in the release of gonadotropin. Herring is the most phylogenetically ancient animal in which three forms of GnRH have been isolated and sequenced. Our evidence suggests that the existence of three GnRHs in the brain of one species 1) is an ancestral condition for teleosts, 2) has the potential for separate regulation of the distinct GnRHs, and 3) may be an evolutionary advantage for refined control of reproduction in different environments.
- Date created
- License information
- Copyright © 2000 by The Endocrine...
- Citation for previous publication
Carolsfeld, J., Powell, J.F., Park, M., Fischer, W.H., Craig, A.G., Chang, J.P., Rivier, J.E. and Sherwood, N.M. "Primary Structure and Function of Three Gonadotropin-
Releasing Hormones, Including a Novel Form, from an
Ancient Teleost, Herring." Endocrinology 141 (2000): 505-512.
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 233858
Last modified: 2015:10:12 17:11:50-06:00
Original checksum: 5719aac127f99e10b3636860bf1603f8
Well formed: true
File title: ee020000505p
Page count: 8