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Permanent link (DOI): https://doi.org/10.7939/R3MW28S0S

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Pyranopterin Coordination Controls Molybdenum Electrochemistry in Escherichia coli Nitrate Reductase Open Access

Descriptions

Other title
Subject/Keyword
Electrochemistry
respiration
electron potential
Biochemistry
electron paramagnetic resonance
nitrate reductase
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Wu, Sheng Yi
Supervisor and department
Weiner, Joel (Biochemistry)
Examining committee member and department
Young, Howard (Biochemistry)
Chen, Xing-zhen (Physiology)
Lemire, Bernard (Biochemistry)
Department
Department of Biochemistry
Specialization

Date accepted
2015-09-30T14:40:42Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
Molybdenum is an essential trace element for most species on the earth. Molybdoenzymes (enzymes containing Molybdenum) play diverse roles in human health, global geochemical cycles, and bacterial metabolism. Interestingly, nitrate reductase (NarGHI) isolated from the common gut bacterium Escherichia coli is an ideal model to study how Mo functions. Molybdenum does not act alone but is held in molybdoenzymes by a complex organic molecule called molybdenum cofactor. I studied the molybdenum cofactor in NarGHI. My research addressed the role of amino acids in the protein that interact with the molybdenum cofactor and the result suggested that the organic component of the cofactor plays a pivotal role in controlling enzyme activity. This research offers a broad implication in the field of molybdoenzyme research and can potentially benefit drug development for molybdoenzyme-related diseases.
Language
English
DOI
doi:10.7939/R3MW28S0S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Wu, Sheng-Yi, Rothery, Richard A., Weiner, Joel H. Pyranopterin Coordination Controls Molybdenum Electrochemistry in Escherichia coli Nitrate Reductase. Journal of Biological Chemistry.(2015). doi:10.1074/jbc.M115.665422

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