ERA

Download the full-sized PDF of Genetic and Hormonal Regulation of Stem Vascular Tissue Development In Flax (Linum usitatissimum L.)Download the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3DK6Q

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Genetic and Hormonal Regulation of Stem Vascular Tissue Development In Flax (Linum usitatissimum L.) Open Access

Descriptions

Other title
Subject/Keyword
linseed, flax, bast fibre, xylem, gibberellin, auxin, gene, SSR
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
McKenzie, Ryan
Supervisor and department
Deyholos, Michael (Biological Sciences)
Examining committee member and department
Hall, Linda (Agriculture, Food and Nutritional Science)
Cooke, Janice (Biological Sciences)
Schultz, Elizabeth (Biological Sciences, University of Lethbridge)
Ozga, Jocelyn (Agriculture, Food and Nutritional Science)
Department
Department of Biological Sciences
Specialization

Date accepted
2011-06-23T15:23:17Z
Graduation date
2011-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Flax (Linum usitatissimum) has been grown as a source of oil and fibre for several millennia. Linseed flax varieties are cultivated for their seed (flaxseed) and for their seed oils (linseed oil), which are used for many industrial applications. The fibres that form in linseed straws are generally considered too poor to make these industrial uses economical. In order to better understand how the flax stem and its component fibres develop, the effects of two plant hormones, gibberellin (GA) and auxin (indole-3-acetic acid, IAA), on stem tissue properties were examined. GA levels were determined to be a particularly important factor in many aspects of linseed stem development, including bast fibre cellular elongation and expansion. The spatial, temporal and hormonal-responsive expression patterns of five genes putatively involved in GA response (LuGAST1), GA biosynthesis (LuGA2ox1, LuGA3ox1), IAA response (LuIAA1) and IAA transport (LuPIN1) were also examined. A potential association of increases in LuGAST1 transcription with the cessation of bast fibre elongation and onset of secondary cell wall biosynthesis suggested a potential involvement of LuGAST1 in these processes. Through a mutant screen of an elite linseed cultivar, a novel mutant was identified, reduced fibre1 (rdf), which lacks a normal complement of fully differentiated fibres in its stem. The preliminary characterization of rdf shows that RDF function may be required for bast fibre elongation. Polymorphic simple sequence repeat (SSR) markers were also identified which will be useful in future for facilitating the cloning and sequencing of RDF through map-based cloning.
Language
English
DOI
doi:10.7939/R3DK6Q
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-29T16:25:24.188+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 10847829
Last modified: 2015:10:12 19:00:42-06:00
Filename: Full Thesis -- RYAN -- June 14 2011.pdf
Original checksum: de626fa6eed6b6424408576646c7cf72
Well formed: true
Valid: true
File title: Microsoft Word - Full Thesis -- RYAN -- June 14 2011.doc
File author: ryan.mckenzie
Page count: 270
Activity of users you follow
User Activity Date