Download the full-sized PDF of The Adhesion of Stored Red Blood Cells to Human Umbilical Vein Endothelial CellsDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

The Adhesion of Stored Red Blood Cells to Human Umbilical Vein Endothelial Cells Open Access


Other title
human umbilical vein endothelial cells
red blood cell adherence
Type of item
Degree grantor
University of Alberta
Author or creator
Nunes, Julien
Supervisor and department
Acker, Jason (Laboratory Medicine and Pathology)
Holovati, Jelena (Laboratory Medicine and Pathology)
Examining committee member and department
Keelan, Monika (Laboratory Medicine and Pathology)
Ward, Chris (Laboratory Medicine and Pathology)
Onell, Rodrigo (Laboratory Medicine and Pathology)
Medical Sciences- Laboratory Medicine and Pathology

Date accepted
Graduation date
Master of Science
Degree level
The field of transfusion medicine is dependent upon the ability to store and preserve the native functionality of red blood cells (RBCs). The subtle membrane changes that the RBC incurs during storage can result in compromised quality and diminished function upon transfusion. A custom adhesion assay was developed which was used to determine the change in red blood cell-endothelial (RBC-EC) interactions in cryopreserved and hypothermic RBCs. This thesis demonstrated an increase in RBC adherence and adherence strength due to the hypothermic storage lesion and cryoinjury. Through these analytical investigations, this thesis has provided a clearer understanding of RBC-EC interactions and have enhanced our understanding of the mechanisms involved in RBC-EC interactions. The resulting benefit of this investigation’s findings should result in a better quality product being available for transfusion which will thus benefit the field of biopreservation and transfusion medicine.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1271134
Last modified: 2015:10:12 14:17:40-06:00
Filename: Nunes_Julien_Spring 2013.pdf
Original checksum: a3f441d82ba7db963be2db8574c7d2c5
Well formed: false
Valid: false
Status message: Invalid page tree node offset=729738
Status message: Unexpected error in findFonts java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary offset=2998
Status message: Invalid Annotation list offset=1200602
Status message: Outlines contain recursive references.
File title: Chapter 1
File language: EN-CA
Activity of users you follow
User Activity Date