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Permanent link (DOI): https://doi.org/10.7939/R3S984

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The Adhesion of Stored Red Blood Cells to Human Umbilical Vein Endothelial Cells Open Access

Descriptions

Other title
Subject/Keyword
human umbilical vein endothelial cells
red blood cell adherence
deformability
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Nunes, Julien
Supervisor and department
Acker, Jason (Laboratory Medicine and Pathology)
Holovati, Jelena (Laboratory Medicine and Pathology)
Examining committee member and department
Keelan, Monika (Laboratory Medicine and Pathology)
Ward, Chris (Laboratory Medicine and Pathology)
Onell, Rodrigo (Laboratory Medicine and Pathology)
Department
Medical Sciences- Laboratory Medicine and Pathology
Specialization

Date accepted
2013-01-30T13:11:44Z
Graduation date
2013-06
Degree
Master of Science
Degree level
Master's
Abstract
The field of transfusion medicine is dependent upon the ability to store and preserve the native functionality of red blood cells (RBCs). The subtle membrane changes that the RBC incurs during storage can result in compromised quality and diminished function upon transfusion. A custom adhesion assay was developed which was used to determine the change in red blood cell-endothelial (RBC-EC) interactions in cryopreserved and hypothermic RBCs. This thesis demonstrated an increase in RBC adherence and adherence strength due to the hypothermic storage lesion and cryoinjury. Through these analytical investigations, this thesis has provided a clearer understanding of RBC-EC interactions and have enhanced our understanding of the mechanisms involved in RBC-EC interactions. The resulting benefit of this investigation’s findings should result in a better quality product being available for transfusion which will thus benefit the field of biopreservation and transfusion medicine.
Language
English
DOI
doi:10.7939/R3S984
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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