Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R37H87
This file is in the following communities:
|Graduate Studies and Research, Faculty of|
This file is in the following collections:
|Theses and Dissertations|
Optimizing impermeant support in an intraluminal preservation solution tailored to the small intestine Open Access
- Other title
- Type of item
- Degree grantor
University of Alberta
- Author or creator
- Supervisor and department
Dr. Thomas A. Churchill (Department of Surgery)
- Examining committee member and department
Dr. Thomas Mueller (Department of Medicine)
Dr. Rachel G. Khadaroo (Department of Surgery)
Department of Surgery
- Date accepted
- Graduation date
Master of Science
- Degree level
The AA (amino acid) solution is an experimental preservation solution delivered intraluminally and tailored to the metabolic requirements of the small bowel. Preservation solutions contain impermeant molecules to prevent edema formation. Dextrans and hydroxyethylstarches are two such oncotic agents. The effectiveness of these molecules has never been compared, nor has the appropriate concentration ever been established within the AA solution. Rat intestines were harvested after an intravascular flush with University of Wisconsin (UW) solution and flushed intraluminally with UW or AA solution containing either dextran70 (D70; MW= 70 kDa) or hydroxyethylstarch (HES; MW= 2,200 kDa). Fluorescent microscopy, energetics, oxidative stress, and histology revealed the superiority of the HES-supplemented AA solution. In a subsequent experiment, tissues were preserved with AA solution containing 0, 2.5, 5, or 10% HES. Energy parameters and histology were superior in the 5% group. Together, these studies support the use of 5% HES within the AA solution.
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
- Citation for previous publication
Cryobiology. 2010 Dec;61(3):254-62. Epub 2010 Sep 21.
Comparing the effects of Dextran 70 and Hydroxyethyl starch in an intestinal storage solution.
Kokotilo MS, Schlachter K, Carter J, Thiesen A, Khadaroo RG, Churchill TACryobiology. 2010 Dec;61(3):236-42. Epub 2010 Aug 19.
Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution.
Kokotilo MS, Carter J, Thiesen A, Chen MH, Ochs A, Khadaroo RG, Churchill TA.
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1982581
Last modified: 2015:10:12 16:54:55-06:00
Filename: Kokotilo_Matthew Fall 2012.pdf
Original checksum: c62f1fae42f4bcceee769abdd2df7c50
Well formed: true
Status message: File header gives version as 1.4, but catalog dictionary gives version as 1.3
Page count: 150