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Optimizing impermeant support in an intraluminal preservation solution tailored to the small intestine Open Access


Other title
Small Intestine
Type of item
Degree grantor
University of Alberta
Author or creator
Kokotilo, Matthew
Supervisor and department
Dr. Thomas A. Churchill (Department of Surgery)
Examining committee member and department
Dr. Thomas Mueller (Department of Medicine)
Dr. Rachel G. Khadaroo (Department of Surgery)
Department of Surgery
Experimental Surgery
Date accepted
Graduation date
Master of Science
Degree level
The AA (amino acid) solution is an experimental preservation solution delivered intraluminally and tailored to the metabolic requirements of the small bowel. Preservation solutions contain impermeant molecules to prevent edema formation. Dextrans and hydroxyethylstarches are two such oncotic agents. The effectiveness of these molecules has never been compared, nor has the appropriate concentration ever been established within the AA solution. Rat intestines were harvested after an intravascular flush with University of Wisconsin (UW) solution and flushed intraluminally with UW or AA solution containing either dextran70 (D70; MW= 70 kDa) or hydroxyethylstarch (HES; MW= 2,200 kDa). Fluorescent microscopy, energetics, oxidative stress, and histology revealed the superiority of the HES-supplemented AA solution. In a subsequent experiment, tissues were preserved with AA solution containing 0, 2.5, 5, or 10% HES. Energy parameters and histology were superior in the 5% group. Together, these studies support the use of 5% HES within the AA solution.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Cryobiology. 2010 Dec;61(3):254-62. Epub 2010 Sep 21. Comparing the effects of Dextran 70 and Hydroxyethyl starch in an intestinal storage solution. Kokotilo MS, Schlachter K, Carter J, Thiesen A, Khadaroo RG, Churchill TACryobiology. 2010 Dec;61(3):236-42. Epub 2010 Aug 19. Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. Kokotilo MS, Carter J, Thiesen A, Chen MH, Ochs A, Khadaroo RG, Churchill TA.

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