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Characterization and silencing of differentially abundant proteins from Pyrenophora tritici-repentis
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- Author / Creator
- Fu, Heting
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Tan spot, caused by Pyrenophora tritici-repentis, is an important foliar disease of wheat. Three genes (stp, unp and glu) encoding proteins previously found to be more abundant in pathogenic versus non-pathogenic fungal isolates were cloned, characterized, heterologously expressed, and silenced with the RNA silencing vector pSilent-1. The unp gene encoded a 16.9 kDa protein belonging to a superfamily of glycine-rich RNA-binding proteins, but growth and virulence of an unp-silenced strain of the fungus were not significantly different from the wild-type. The stp gene, which was not significantly silenced, encoded a 15.0 kDa protein homologous to a CipC-like antibiotic response protein. The glu gene encoded an exo-1,3-β-glucanase, 46.7 kDa in mass. Four glu-silenced strains were obtained. The strain in which silencing was strongest exhibited reduced growth, produced fewer appressoria, and caused less disease than the wild-type. The results suggest that exo-1,3-β-glucanase contributes to the development and virulence of P. tritici-repentis.
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- Subjects / Keywords
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- Graduation date
- Fall 2011
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.