ERA

Download the full-sized PDF of Characterization of lipooligosaccharide as a phage receptor and fitness factor in Burkholderia cenocepaciaDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3J38KR94

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Characterization of lipooligosaccharide as a phage receptor and fitness factor in Burkholderia cenocepacia Open Access

Descriptions

Other title
Subject/Keyword
PC184
Phage Therapy
Bacteriophages
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Abdu, Ashraf H
Supervisor and department
Dr. Jonathan Dennis (Biological Sciences)
Examining committee member and department
Lisa Stein (Biological Sciences)
Christine Szymanski (Biological Sciences)
Stefan Pukatzki (Medical Microbiology & Immunology)
Department
Department of Biological Sciences
Specialization
Microbiology and Biotechnology
Date accepted
2013-08-29T10:45:17Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The Burkholderia cepacia complex (Bcc) are ubiquitous in nature and can be isolated from soil, water, plants and more recently in hospitals. The Bcc comprises at least 17 Gram-negative bacterial species that are notorious for their pathogenic effects in immunocompromised individuals. Host colonization by these bacteria causes problems due to their innate resistance to antibiotics. Because of the difficulties in treating Bcc infections, researchers are reevaluating bacteriophages as an alternative therapy to antibiotics. My objective was to confirm the receptor for the Bccspecific bacteriophage KS10, as this knowledge will allow for the construction of effective multi-receptor phage cocktails to treat Bcc infections. Through the use of an updated I-SceI deletion system, we were able to construct LOS mutants to verify previous findings that KS10 interacts with LOS as a receptor. In addition to KS10 resistance, both wabO and waaC mutations displayed reduced fitness in the form of decreased growth and swimming motility when compared to wildtype PC184. These results suggest that phage-resistant mutations can be detrimental to the bacteria.
Language
English
DOI
doi:10.7939/R3J38KR94
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-29T15:49:00.710+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 20847896
Last modified: 2015:10:12 16:37:33-06:00
Filename: Ashraf Abdu Thesis-Revisions.pdf
Original checksum: 765abb7eaea9b94123da9d67835ee364
Well formed: true
Valid: true
Status message: File header gives version as 1.4, but catalog dictionary gives version as 1.3
Status message: Too many fonts to report; some fonts omitted. Total fonts = 1232
File title: Microsoft Word - Ashraf Abdu Thesis-Revisions.docx
File author: Ashraf Abdu
Page count: 148
Activity of users you follow
User Activity Date