Download the full-sized PDF of Cellular mechanisms of ion and acid-base transport in aquatic animalsDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

Cellular mechanisms of ion and acid-base transport in aquatic animals Open Access


Other title
cellular imaging
mitochondrion-rich cells
Type of item
Degree grantor
University of Alberta
Author or creator
Parks, Scott Kenneth
Supervisor and department
Goss, Greg (Biological Sciences)
Examining committee member and department
McCormick, Stephen (Biology, University of Massachusetts Amherst)
Duszyk, Marek (Physiology)
Leys, Sally (Biological Sciences)
Ali, Declan (Biological Sciences)
Department of Biological Sciences
Physiology, Cell and Developmental Biology
Date accepted
Graduation date
Doctor of Philosophy
Degree level
I investigated cellular mechanisms of ion and acid-base transport in rainbow trout (Oncorhyncus mykiss), crabs (Neohelice granulata), zebrafish (Danio rerio), Pacific hagfish (Eptatretus stoutii), and mosquito larvae (Aedes aegypti) with a primary focus on discerning the mechanisms governing ion transport and acid base regulation. In rainbow trout I provide the first functional evidence for two physiologically distinct mitochondrion-rich (MR) cells at the gill and demonstrate a new model for transepithelial Na+ uptake from freshwater involving apical Na+ channels and basolateral Na+/HCO3- co-transporters. These data are supported by extensive thermodynamic consideration of Na+ uptake from freshwater. I also demonstrate functional Cl-/HCO3- exchangers in both MR cell subtypes with roles for Cl- uptake and intracellular pH (pHi) regulation respectively and I present the first evidence for a Cl- dependent Na+/H+ exchanger in gill MR cells. Finally I demonstrate a unique Na+ dependent pHi recovery mechanism that requires protein kinase C for activation. A major limiting factor in clarifying the mechanisms of Na+ uptake in freshwater fish is the lack of a typical Na+ channel in any of the fish molecular databases. My work on zebrafish, although preliminary, indicates that a member of the acid-sensing ion channel family could be responsible for Na+ uptake from freshwater. I then expanded my research outside the trout model using an isolated crab gill preparation. I provide a cellular model for H+ secretion in crab gills that supports the transepithelial Na+ transport model that I described in rainbow trout. In Pacific hagfish, I demonstrate that recovery from blood acidosis is dependent on a Na+/H+ exchanger in gill MR cells. This mechanism of regulation involves translocation from the cytoplasm to the apical membrane during acidotic stress. This data combines with other studies demonstrating the mechanisms of acid and base secretion from a single MR cell subtype. Finally, I show that serotonin stimulation alkalinizes the pHi of the anterior midgut cells in the larval mosquito to levels never before observed in cell biology. These data challenge the dogma of pHi regulation in cell biology and demonstrate the power of using a comparative approach to systems physiology.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 4374354
Last modified: 2015:10:12 20:05:30-06:00
Filename: Parks_Scott_Fall2009.pdf
Original checksum: 7b7985d3a5bcc6200df695d949277515
Well formed: true
Valid: true
Status message: Too many fonts to report; some fonts omitted. Total fonts = 1580
File title: Microsoft Word - Parks SK PhD thesis Revisions _final version_.doc
File author: Greg
Page count: 392
Activity of users you follow
User Activity Date