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Alternative Requirements for Vestigial, Scalloped, and Dmef2 during Muscle Differentiation in Drosophila melanogaster Open Access

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Author or creator
Deng, H.
Hughes, S. C.
Bell, J. B.
Simmonds, A. J.
Additional contributors
Subject/Keyword
Cell-differentiation
Target promoters
MEF2 transcription factors
Flight-muscle
Mads box
Cardiac development
Wing formation
Enhancer factor-I
Gene-expression
Tropomyosin gene
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Abstract: Vertebrate development requires the activity of the myocyte enhancer factor 2 (mef2) gene family for muscle cell specification and subsequent differentiation. Additionally, several muscle-specific functions of MEF2 family proteins require binding additional cofactors including members of the Transcription Enhancing Factor-1 (TEF-1) and Vestigial-like protein families. In Drosophila there is a single mef2 (Dmef2) gene as well single homologues of TEF-1 and vestigial-like, scalloped (sd), and vestigial (vg), respectively. To clarify the role(s) of these factors, we examined the requirements for Vg and Sd during Drosophila muscle specification. We found that both are required for muscle differentiation as loss of sd or vg leads to a reproducible loss of a subset of either cardiac or somatic muscle cells in developing embryos. This muscle requirement for Sd or Vg is cell specific, as ubiquitous overexpression of either or both of these proteins in muscle cells has a deleterious effect on muscle differentiation. Finally, using both in vitro and in vivo binding assays, we determined that Sd, Vg, and Dmef2 can interact directly. Thus, the muscle-specific phenotypes we have associated with Vg or Sd may be a consequence of alternative binding of Vg and/or Sd to Dmef2 forming alternative protein complexes that modify Dmef2 activity.
Date created
2009
DOI
doi:10.7939/R3PG1HP89
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This version of this article is open access and can be downloaded and shared. This Creative Commons Attribution-NonCommercial-ShareAlike 3.0 license lets others remix, tweak, and build upon your work non-commercially, as long as they credit you and license their new creations under the identical terms. The original author(s) and source must be cited.
Citation for previous publication
Deng, H., Hughes, S. C., Bell, J. B., & Simmonds, A. J. (2009). Alternative Requirements for Vestigial, Scalloped, and Dmef2 during Muscle Differentiation in Drosophila melanogaster. Molecular Biology of the Cell, 20(1), 256-269. DOI: 10.1091/mbc.E08-03-0288.
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