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Permanent link (DOI): https://doi.org/10.7939/R3S636

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Mapping and characterization of mel-43(sb41), a gene required for early embryonic viability in C. elegans Open Access

Descriptions

Other title
Subject/Keyword
embryo
mel-43(sb41)
polarity
elegans
Caenorhabditis
maternal-effect
meiosis
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Curtis Pahara, Donna
Supervisor and department
Srayko, Martin (Biological Sciences)
Examining committee member and department
Frank Nargang (Biological Sciences)
Nicolas Touret (Biochemistry)
Department
Department of Biological Sciences
Specialization

Date accepted
2010-01-04T19:54:43Z
Graduation date
2010-06
Degree
Master of Science
Degree level
Master's
Abstract
A genetic screen for dominant, temperature-sensitive, maternal-effect embryonic lethal mutations identified mel-43(sb41), a gene required for early embryonic viability (Mitenko et al., 1997). Linkage mapping placed mel-43 within a small region on chromosome IV. Genetic analyses suggested that mel-43(sb41) was a neomorphic mutation. While refining the genetic position of the mel-43 gene, data suggested that the genetic position of mel-43 was inconsistent with the published location. In light of this new location, previous conclusions regarding the genetic behaviour of mel-43(sb41) were re-examined. Deficiency analysis suggests that mel-43(sb41) is a haploinsufficient loss-of-function mutation. mel-43(sb41) embryos are significantly delayed in meiosis II independent of cyclin B1 degradation. Consequently, embryos fail to produce meiosis II polar bodies and do not establish proper polarity. Although the function of mel-43 remains unknown, the persistent meiotic spindle suggests that mel-43 acts upstream of the microtubule rearrangements necessary to promote the metaphase II to anaphase II transition.
Language
English
DOI
doi:10.7939/R3S636
Rights
License granted by Donna Curtis Pahara (dc4@ualberta.ca) on 2009-12-29T14:48:28Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File format: pdf (Portable Document Format)
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File size: 17028932
Last modified: 2015:10:12 12:57:18-06:00
Filename: CurtisPahara_Donna_Spring 2010.pdf
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Status message: File header gives version as 1.4, but catalog dictionary gives version as 1.3
File title: Microsoft Word - alternative.docx
File author: Donna Curtis
Page count: 112
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