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Engineering nitrogen use efficiency in Oryza sativa by the developmental over-expression of barley alanine aminotransferase using a novel rice promoter Open Access


Other title
Nitrogen use efficiency
Oryza sativa
Alanine aminotransferase
Aldehyde dehydrogenase
Type of item
Degree grantor
University of Alberta
Author or creator
Lock, Yee Ying
Supervisor and department
Good, Allen (Biological Sciences)
Examining committee member and department
Scarpella, Enrico (Biological Sciences)
Kav, Nat (Agriculture, Life and Environmental Sciences)
Department of Biological Sciences

Date accepted
Graduation date
Master of Science
Degree level
Nitrogen is the main limiting component to plant growth and development. With increasing cost of food production the development of nitrogen use efficient (NUE) crop plants has become of interest. In our group, the over-expression of barley alanine aminotransferase (HvAlaAT) cDNA by the btg26 and OsANT1 promoters has yielded NUE plants. Through a detailed bioinformatic analysis, the PBpr1 promoter was selected, cloned, and coupled to HvAlaAT for over-expression in rice (Oryza sativa cv. Nipponbare). Overall, rice lines carrying PBpr1::HvAlaAT resulted in higher seed yield, biomass and tillering compared to non-transformed Nipponbare. Molecular analysis of these lines showed high levels of HvAlaAT mRNA and alanine aminotransferase (AlaAT) protein. Transgenic shoots exhibits high levels of AlaAT activity at all times while root activity increased only after active tillering, indicating developmental regulation of the gene in roots. Analysis of PBpr1 pattern expression, however, showed no definitive staining despite the observed over-expression of HvAlaAT at the transcript and enzyme activity level.
License granted by Yee Ying Lock ( on 2011-09-13T17:57:34Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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