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2012

Vocadlo, David J. , Chan, Jefferson, Whitworth, Garrett A., Macauley, Matthew S., Stubbs, Keith A., Varki, Ajit, Davies, Gideon J., Yuzwa, Scott, Zandberg, Wesley, Bennet, Andrew J., He, Yuan

The O-GlcNAc modification involves the attachment of single β-O-linked N-acetylglucosamine residues to serine and threonine residues of nucleocytoplasmic proteins. Interestingly, previous biochemical and structural studies have shown that O-GlcNAcase (OGA), the enzyme that removes O-GlcNAc from...

2017-01-01

Bernard, G.M., Goyal, A., Miskolzie, M., McKay, R., Wu, Q., Wasylishen, R.E., Michaelis, V.K.

A new solid-state nuclear magnetic resonance (NMR) thermometry sample is proposed. The 207Pb NMR chemical shift of a lead halide perovskite, methylammonium lead chloride (MAPbCl3) is very sensitive to temperature, 0.905 ± 0.010 ppm K−1. The response to temperature is linear over a wide...

2013-01-01

Islam, Molla R., Johnson, Kai C. C., Serpe, Michael J.

Poly (N-isopropylacrylamide)-co-acrylic acid (pNIPAm-co-AAc) microgels were “painted” on the Au electrode of a quartz crystal microbalance (QCM). Another Au layer (overlayer) was subsequently deposited on the microgel layer. This structure is known as a microgel-based etalon. These devices have...

2018-07-01

Chao Wang, Mona Amiri, Riley T. Endean, Octavio Martinez Perez, Samuel Varley, Ben Rennie, Loorthuraja Rasu, Steven H. Bergens

1,10-phenanthroline is grafted to indium tin oxide (ITO) and titanium dioxide nanoparticle (TiO2) semiconductors by electroreduction of 5-diazo-1,10-phenanthroline in 0.1 M H2SO4. The lower and upper potential limits (-0.20 and 0.15 VSCE, respectively) were set to avoid reduction and oxidation of...

2018-05-16

Richards, Michele R, Guo, Tianlin, Hunter, Carmanah D, Cairo, Christopher W.

Inhibitors of viral neuraminidase enzymes have been previously developed as therapeutics. Humans can express multiple forms of neuraminidase enzymes (NEU1, NEU2, NEU3, NEU4) that share a similar active site and enzymatic mechanism with their viral counterparts. Using a panel of purified human...

2017-01-01

Zhang, Qiang, Colazo, Juan, Berg, Darren, Mugo, Samuel M., Serpe, Michael J.

Nanogels with a biomolecular coating (biocoating) were shown to be capable of triggered delivery of anticancer drug Doxorubicin. The biocoating was formed utilizing binding between glycogen and the tetra-functional lectin Concanavalin A, which can be triggered to disassemble (and release) upon...

2017-01-01

Askar, A.,, Bernard, G.M, Wiltshire,, B., Shankar, K., Michaelis, V.K.

An NMR investigation of methylammonium lead iodide, the leading member of the hybrid organic-inorganic perovskite class of materials, and of its putative decomposition products as a result of exposure to heat and humidity, has been undertaken. We show that the 207Pb NMR spectra of the compound of...

2013-01-01

Nair, Sindhu G., Loppnow, Glen R.

The exposure of DNA to ultraviolet (UV) radiation causes sequence‐dependent damage. Thus, there is a need for an analytical technique that can detect damage in large numbers of DNA sequences simultaneously. In this study, we have designed an assay for UVC‐induced DNA damage in multiple...

2014-01-01

Wang, Jingxia, Hu, Liang, Song, Yanlin, Serpe, Michael J.

Janus poly (N-isopropylacrylamide)-co-acrylic acid/Au microgels that resemble a “snowman”, “dumbbell”, and an “abalone” were prepared by thermally evaporating a layer of Au on half of the microgel surface, followed by exposure to thiolated single-stranded DNA with complementary sequences. We...

2010

Kitova, Elena N., Sun, Nian, Klassen, John S., Soya, Naoto

An investigation of the nonspecific association of small charged biomolecules and proteins in electrospray ionization mass spectrometry (ES-MS) is described. Aqueous solutions containing pairs of proteins and a small acidic or basic biomolecule that does not interact specifically with either of...