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Development and Applications of Chemical Isotope Labeling Methods for Metabolome Sample Normalization and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadFall 2015
Metabolomics refers to the global study of the metabolome of a biological system where comprehensive and systematic profiling of all metabolites in a given biological sample (i.e., the metabolome) is performed. As the end product of biological processes, the status of metabolites could reflect...
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Development of Isotope Labeling Liquid Chromatography Mass Spectrometry for Biofluid Metabolomics and Applications in Disease Studies
DownloadSpring 2014
Metabolomics is a research field focusing on global study of all the metabolites present in a biological system (i.e., the metabolome). Metabolome analysis involves identifying and quantifying as many small molecule metabolites as possible in a biological sample. Metabolomics has attracted much...
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Development of Isotope Labeling Methods and Data Processing Program for Liquid Chromatography Mass Spectrometry-Based Metabolome Analysis
DownloadSpring 2014
Metabolomics strives to gain a comprehensive picture of the metabolites in a biological system. This emerging research field gives us an insight into the discovery of disease-related biomarkers and an understanding of metabolic flux as well as mechanisms. Liquid chromatography mass spectrometry...
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Development of New Late-Stage Labeling Methods with Labeled Carbon for Stabilized Carboxylic Acids and α-Amino Acids
DownloadFall 2023
Isotope labeling of small molecules is a crucial tool for drug discovery and understanding biochemical processes. Expanding the toolbox of a radiochemist with methods that allow late-stage labeling is therefore important. Of the methods that have been developed, the use of isotopically labeled...