SearchSkip to Search Results
- 1Braithwaite, Shannon Lynn
- 1Coltman, D.W.
- 1Hodgetts, Bill
- 1Hopper, Tammy
- 1Ickert, Carla
- 1Kausar, Abu
A repetitive element of approximately 200 bp was cloned from harbour seal (Phoca vitulina concolour) genomic DNA. The sequence of the element revealed putative RNA polymerase III control boxes, a poly A tail and direct terminal repeats characteristic of SINEs. Sequence and secondary structural...
Hearing loss and cognitive-communication test performance of long-term care residents with dementia: Effects of amplificationDownload
Purpose: The study aims were: 1) to explore the relationship between hearing loss and cognitive-communication performance of individuals with dementia, and 2) to determine if hearing loss is accurately identified by long-term care (LTC) staff. The research questions were: 1) What is the effect...
Mitochondrial DNA variation and identification of bark weevils in the Pissodes strobi species group in western Canada (Coleoptera: Curculionidae)Download
Abstract: Morphological, allozyme, and chromosomal characters and ecological traits have limited value for discriminating among four closely related Pissodes spp. known from western Canada. We amplified a 1585-bp segment of mitochondrial DNA (mtDNA), including half of the cytochrome oxidase I...
Optimization, adaptation and application of protein misfolding cyclic amplification to detection of prions in blood plasmaDownload
The PMCA assay was optimized for adaptation to low level detection of PrPSc in hamster plasma. Evaluation of numerous key variables of the PMCA assay led to an optimized protocol capable of ~3 log10 amplification after 32 cycles (two 16 hour rounds). When commercially purchased normal hamster...
Developing a simple, general, and isothermal self-replicating system is one of the key requirements for a simplified detection platform for nucleic acid sequences specific to a disease causing microrganism. One of the challenges in achieving nucleic-acid templated isothermal amplification is that...