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Investigating cryoinjury using simulations and experiments: 1. TF-1 cells during two-step freezing (rapid cooling interrupted with a hold time)Download
There is significant interest in designing a cryopreservation protocol for hematopoietic stem cells (HSC) which does not rely on dimethyl sulfoxide (Me2SO) as a cryoprotectant. Computer simulations that describe cellular osmotic responses during cooling and warming can be used to optimize the...