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- 8Cairo, Christopher W
- 4Guo, Tianlin
- 4Richards, Michele R
- 3Hunter, Carmanah D
- 3Zou, Chunxia
- 2Daskhan, Gour
- 1Affinity chromatography
- 1Carbohydrate chemistry
- 1Cell biology
Construction of multivalent homo- and hetero-functional ABO blood group glycoconjugates using a trifunctional linker strategy
Construction of multivalent homo- and hetero-functional ABO blood group glycoconjugates using a trifunctional linker strategyDownload
The design and synthesis of multivalent ligands displaying complex oligosaccharides is necessary for the development of therapeutics, diagnostics, and research tools. Here, we report an efficient conjugation strategy to prepare complex glycoconjugates with four copies of one or two separate...
Human neuraminidase isoenzymes show variable activities for 9-O-acetyl-sialoside substratesDownload
Recognition of terminal sialic acids is central to many cellular processes, and structural modification of sialic acid can disrupt these interactions. A prominent, naturally occuring, modification of sialic acid is 9-O-acetylation (9-O-Ac). Study of this modification through generation and...
Integrins are critical receptors in cell migration and adhesion. A number of mechanisms are known to regulate the function of integrins, including phosphorylation, conformational change, and cytoskeletal anchoring. We investigated whether native neuraminidase (Neu, or sialidase) enzymes which...
Molecular dynamics simulations of viral neuraminidase inhibitors with the human neuraminidase enzymes: Insights into isoenzyme selectivity
Molecular dynamics simulations of viral neuraminidase inhibitors with the human neuraminidase enzymes: Insights into isoenzyme selectivityDownload
Inhibitors of viral neuraminidase enzymes have been previously developed as therapeutics. Humans can express multiple forms of neuraminidase enzymes (NEU1, NEU2, NEU3, NEU4) that share a similar active site and enzymatic mechanism with their viral counterparts. Using a panel of purified human...
Protecting group-free immobilization of glycans for affinity chromatography using glycosylsulfonohydrazide donorsDownload
A variety of applications in glycobiology exploit affinity chromatography through the immobilization of glycans to a solid support. Although several strategies are known, they may provide certain advantages or disadvantages in how the sugar is attached to the affinity matrix. Additionally, the...
Selective Inhibitors of Human Neuraminidase 3
Human neuraminidases (NEU) are associated with human diseases including cancer, atherosclerosis, and diabetes. To obtain small molecule inhibitors as research tools for the study of their biological functions, we designed a library of 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA) analogues...
Selective inhibitors of human neuraminidase 1 (NEU1)
Inhibitors of human neuraminidase enzymes (NEU) are recognized as important tools for the study of the biological functions of NEU and will be potent tools for elucidating the role of these enzymes in regulating the repertoire of cellular glycans. Here we report the discovery of selective...
Synthetic strategies for modified glycosphingolipids and their design as probes
The plasma membrane of cells contains a diverse array of lipids that provide important structural and biological features. Glycolipids are typically a minor component of the cell membrane and consist primarily of glycosphingolipids (GSLs). GSLs in vertebrates contain a multifarious assortment of...