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- 69Klassen, John S.
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- 17Serpe, Michael J.
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- 20Electrospray ionization mass spectrometry
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A Convergent Route to Enantiomers of the Bicyclic Monosaccharide Bradyrhizose Leads to Insight into the Bioactivity of an Immunologically Silent Lipopolysaccharide
Download2019-01-27
Claude Larrivée Aboussafy, Lotte Bettina Anderson Gersby, Antonio Molinaro, Mari-Anne Newman, Todd L. Lowary
The synthesis of bradyrhizose, the monosaccharide component of the lipopolysaccharide O-antigen of the nitrogen-fixing bacteria Bradyrhizobium sp. BTAi1 and sp. ORS278, has been achieved in 25 steps in an overall yield of 6% using myo-inositol and ethyl propiolate as the starting materials. The...
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2018-11-16
Guo, Tianlin, Heon-Roberts, Rachel, Zou, Chunxia, Zheng, Ruixiang, Pshezhetsky, Alexey V, Cairo, Christopher W.
Inhibitors of human neuraminidase enzymes (NEU) are recognized as important tools for the study of the biological functions of NEU and will be potent tools for elucidating the role of these enzymes in regulating the repertoire of cellular glycans. Here we report the discovery of selective...
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2018-01-05
Guo, Tianlin, Datwyler, Philipp, Demina, Ekaterina, Richards, Michele R, Ge, Peng, Zou, Chunxia, Zheng, Ruixiang Blake, Fougerat, Anne, Pshezhetsky, Alexey V, Ernst, Beat, Cairo, Christopher W.
Human neuraminidases (NEU) are associated with human diseases including cancer, atherosclerosis, and diabetes. To obtain small molecule inhibitors as research tools for the study of their biological functions, we designed a library of 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA) analogues...
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Molecular dynamics simulations of viral neuraminidase inhibitors with the human neuraminidase enzymes: Insights into isoenzyme selectivity
Download2018-05-16
Richards, Michele R, Guo, Tianlin, Hunter, Carmanah D, Cairo, Christopher W.
Inhibitors of viral neuraminidase enzymes have been previously developed as therapeutics. Humans can express multiple forms of neuraminidase enzymes (NEU1, NEU2, NEU3, NEU4) that share a similar active site and enzymatic mechanism with their viral counterparts. Using a panel of purified human...
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Modular Construction of Photoanodes with Covalently Bonded Ru- and Ir-Polypyridyl Visible Light Chromophores
Download2018-07-01
Chao Wang, Mona Amiri, Riley T. Endean, Octavio Martinez Perez, Samuel Varley, Ben Rennie, Loorthuraja Rasu, Steven H. Bergens
1,10-phenanthroline is grafted to indium tin oxide (ITO) and titanium dioxide nanoparticle (TiO2) semiconductors by electroreduction of 5-diazo-1,10-phenanthroline in 0.1 M H2SO4. The lower and upper potential limits (-0.20 and 0.15 VSCE, respectively) were set to avoid reduction and oxidation of...
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Human neuraminidase isoenzymes show variable activities for 9-O-acetyl-sialoside substrates
Download2018-01-16
Hunter, Carmanah D, Khanna, Neha, Richards, Michele R, Darestani, Reza Rezaei, Zou, Chunxia, Klassen, John S, Cairo, Christopher W.
Recognition of terminal sialic acids is central to many cellular processes, and structural modification of sialic acid can disrupt these interactions. A prominent, naturally occuring, modification of sialic acid is 9-O-acetylation (9-O-Ac). Study of this modification through generation and...
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Construction of multivalent homo- and hetero-functional ABO blood group glycoconjugates using a trifunctional linker strategy
Download2017-12-12
Daskhan, Gour, Tran, Hanh-Thuc, Meloncelli, Peter J, Lowary, Todd L, West, Lori J, Cairo, Christopher W.
The design and synthesis of multivalent ligands displaying complex oligosaccharides is necessary for the development of therapeutics, diagnostics, and research tools. Here, we report an efficient conjugation strategy to prepare complex glycoconjugates with four copies of one or two separate...
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2017-02-16
Wang, Chao, Moghaddam, Reza B., Bergens, Steven H.
A series of Ir1–xCux (x = 0–0.5) hydrous oxide nanoparticles (HO-np) were prepared simply by stirring solutions of IrCl3 hydrate and CuCl2 hydrate in aqueous KOH under air. Their water oxidation activities were measured in 0.1 M HClO4. The Ir0.89Cu0.11 HO-np was the most active catalyst in the...
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Localizing carbohydrate binding sites in proteins using hydrogen/deuterium exchange mass spectrometry
Download2016
Klassen, John S., Kitova, Elena N., Li, Jun, Zhang, Jingjing, Ng, Kenneth, Eugenio, Luiz
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to localize ligand binding sites in carbohydrate-binding proteins is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin and Shiga toxin type 1 and a fragment of Clostridium...
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Screening anti-cancer drugs against tubulin using catch-and-release electrospray ionization mass spectrometry
Download2016
Kitova, Elena N., Klassen, John S., Winter, Philip, Tuszynski, Jack A., Darestani, Reza R.
Tubulin, which is the building block of microtubules, plays an important role in cell division. This critical role makes tubulin an attractive target for the development of chemotherapeutic drugs to treat cancer. Currently, there is no general binding assay for tubulin–drug interactions. The...