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Development of Blood Metabolomics for Biomarker Discovery Using Chemical Isotope Labeling and Liquid Chromatography-Mass Spectrometry
DownloadSpring 2018
Blood-metabolite-based biomarkers are useful for the early-stage diagnosis, accurate prognostic prediction and personalized treatment of various diseases. Blood contains a massive number of metabolites that can potentially become biomarkers, but the metabolome coverage of current analytical...
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Development and Application of Isotope Labeling Method and Data Processing Program for Liquid Chromatography Mass Spectrometry Based Metabolome Profiling
DownloadSpring 2015
The objective of this thesis focuses on establishing a software tool (IsoMS) used in the quantification and qualification of metabolites in complex biological samples, developing robust methods to extract metabolites from different plant samples, and applying a differential 13C-/12C-isotope...
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...
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Fall 2017
The most often used biofluids in metabolomics and disease biomarker discovery studies in the literature include urine, processed blood (serum or plasma), and cerebral spinal fluid. While investigations using these biofluids have led to numerous innovations in the field of metabolomics, little...
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Development of Chemical Isotope Labeling (CIL) LC-MS Techniques for Untargeted Analysis Milk Metabolomics and Targeted Analysis of Propionic Acid
DownloadFall 2017
Metabolomics is the study of all the small molecules in a given sample or organism. It is used to understand individual variations caused by the relationship between the individual’s genetics, exposome and metabolic activity. There is interest in using metabolomics for personalized care leading...
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Development of Mass Spectrometric Methods for Membrane Proteome Analysis and Protein Sequence Mapping
DownloadSpring 2014
This thesis work mainly focused on the development of mass spectrometric methods for membrane proteome analysis and protein sequence mapping. First, the comparison of the performance of RapiGest-, PPS- and SDS-based sample preparation methods for shotgun membrane proteome analysis was...
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Spring 2021
Lipidomics aims to research lipid metabolism inside various samples or biological organisms. Through the research of lipidomics, information regarding the diversity, abundance, and function of lipids can determined. Although lipids can be categorized into 8 lipid classes, the variability between...
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Development of Data Processing Methods in Chemical Isotope Labeling Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2019
Metabolomics is an active research field on the methods development for the analysis of small metabolites in biological systems. It provides powerful approaches that allow us to examine the variations in the metabolic profiles and is capable of detecting complex biological changes using...
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Lipidomics of Biological Samples for the Assessment of Physiological and Pathological Processes
DownloadFall 2020
Lipids are a large class of hydrophobic molecules involved in energy storage, signaling, modulation of gene expression and membranes. Lipidomics focuses on the comprehensive analysis of lipids and their interactions. Although the potential of lipidomics to study physiological and pathological...
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Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...